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Purification, identification, and characterization of an osmotic response element binding protein.
TLDR
The osmotic response element binding protein (OREBP) is a transcription factor of approximately 200 kDa in size, characterized by a Rel-like DNA binding domain and a glutamine-rich transactivation domain that significantly diminished hyperosmotic AR gene induction.
Overexpression of aldose reductase in liver cancers may contribute to drug resistance
TLDR
HepG2 cells, a stable line of liver cells, were induced to overexpress AR by hypertonicity and cells that were cultured in hypertonic medium became more resistant to daunorubicin, suggesting that overexpression of AR made the cells moreresistant to this drug.
The relative ability of glucose and ascorbate to glycate and crosslink lens proteins in vitro. off.
TLDR
It is argued that glucose crosslinking can be shown in vitro with lens proteins, however, it does not proceed significantly via 3-deoxyglucosone, and does not require transition metal ion-mediated oxidation to occur.
Effect of advanced glycation end products on lens epithelial cells in vitro.
TLDR
The existence of AGEs in human anterior polar lens capsules of cataracts was confirmed using a combination of dot-immunoblot and fluorescent detection and the interaction of LECs with AGE s induced mRNAs and proteins of fibronectin, collagen type I, aberrant extracellular matrix proteins, and alpha-SMA, a specific marker for myofibroblastic cells.
Effects of pore mutations and permeant ion concentration on the spontaneous gating activity of OmpC porin.
TLDR
In order to study the role that electrostatic interactions between pore residues may have in porin function, spontaneous mutants and engineered site-directed mutants that have an altered charge distribution at the eyelet are used and electrophysiological behavior is compared with that of wild-type OmpC.
Chromatographic comparison of the UVA sensitizers present in brunescent cataracts and in calf lens proteins ascorbylated in vitro.
TLDR
It is argued that it is the brown, fluorescent compounds which accumulate during aging and cataract formation that are responsible for the UVA-dependent ROS formation, and that these browning products may be similar to the advanced glycation endproducts produced by ascorbylation of lens proteins under oxidative conditions.
Cloning of CTP:phosphocholine cytidylyltransferase cDNA from Arabidopsis thaliana.
TLDR
The hydropathy profile revealed that the C-terminal non-catalytic portion of the protein was very hydrophilic, highly enriched in negatively charged aspartic acid and glutamic acid residues, and there was an amphipathic alpha-helical domain, which was believed to bind the membrane surface in the active formation.
Cloning of a cDNA encoding phospholipase D from Pimpinella brachycarpa.
TLDR
It was identified that PLD from P. brachycarpa has HQKIVVVD and HAKMMIVD sequences which were homologous with a duplicated HXKXXXXD motif that has been conserved in PLDs from plants, animals, and yeast.
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