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Quantitative RT-PCR: pitfalls and potential.
This review addresses the mathematics of RT-PCR, choice of RNA standards, and quantification strategies (competitive, noncompetitive and kinetic [real-time] amplification) and practical considerations in experimental design.
Intricate Regulation of Tyrosine Hydroxylase Activity and Gene Expression
Regulatory mechanisms provide not only redundancy but also diversity in the control of catecholamine biosynthesis.
Twenty-five years of quantitative PCR for gene expression analysis.
This review examines the current state of qPCR for gene expression analysis now that the method has reached a mature stage of development and implementation and the conceptual framework for data analysis methods is presented to demystify these analysis techniques.
Cytotoxic and genotoxic potential of dopamine
Experimental evidence suggesting that dopamine quinone may contribute to the cytotoxic and genotoxic potential of this essential neurotransmitter is described.
Adenosine cyclic 3',5'-monophosphate dependent protein kinase: kinetic mechanism for the bovine skeletal muscle catalytic subunit.
The kinetic mechanism for adenosine cyclic 3',5'-monophosphate dependent protein kinase was determined from initial velocity studies in the absence and presence of the product MgADP and dead-end inhibitors and the binding of Mg2+ to a second site was characterized.
Circulating Cytokines as Biomarkers of Alcohol Abuse and Alcoholism
This work has shown that circulating cytokines may contribute to diagnostic biomarker panels, and a combination of multiple biomarkers may significantly increase the sensitivity and specificity of the biochemical tests aiding reliable and accurate detection of excessive alcohol intake.
Manganese uptake and distribution in the central nervous system (CNS).
The distribution and membrane transport of Mn emphasizes the importance of: 1) the oxidation state of Mn, as it governs the affinity of Mn to endogenous ligands, and 2) the reaction of Mn3+ with transferrin, the plasma iron-carrying protein.
Parthenogenetic Stem Cells in Nonhuman Primates
No primate parthenogenetic embryonic stem (ES) cell lines have been derived from parthenogenesis in nonhuman primates, but this process has been characterized to some extent in non human primates.
Fundamentals of DNA hybridization arrays for gene expression analysis.
This review will cover the status of the hybridization array field with an eye toward underlying principles and available technologies as well as future developments and technological trends.
Complex molecular regulation of tyrosine hydroxylase
- Izel Tekin, R. Roskoski, N. Carkaci-Salli, K. Vrana
- Biology, ChemistryJournal of Neural Transmission
- 28 May 2014
The myriad mechanisms that regulate tyrosine hydroxylase expression and activity are revisited and their physiological importance in the control of catecholamine biosynthesis is highlighted.