• Publications
  • Influence

Claim Your Author Page
Ensure your research is discoverable on Semantic Scholar. Claiming your author page allows you to personalize the information displayed and manage publications (all current information on this profile has been aggregated automatically from publisher and metadata sources).
  • Vadim E Klyushnichenko, D M Koulich, +4 authors Andrey N Wulfson
  • Chemistry, Medicine
  • Journal of chromatography. A
  • 1994 (First Published: 1 February 1994)
  • The production of recombinant human insulin consists of five main stages, accompanied by considerable transformation of molecules, concerning size, secondary structure and the presence of chargedContinue Reading
  • Vadim E Klyushnichenko, Sergey A Yakimov, Alexander M. Arutyunyan, Alexander E. Ivanov, K. V. Mal'tsev, Andrey N Wulfson
  • Chemistry, Medicine
  • Journal of chromatography. B, Biomedical…
  • 1994 (First Published: 1 December 1994)
  • The applicability of reversed-phase high-performance liquid chromatography (HPLC) to the analysis of the products of recombinant insulin was studied. The influence of several mobile phases inContinue Reading
  • D M Koulich, Igor V. Nazimov, K. V. Mal'tsev, Andrey N Wulfson
  • Chemistry, Medicine
  • Journal of chromatography. B, Biomedical…
  • 1994 (First Published: 1 December 1994)
  • The optimization of the separation of recombinant human insulin (rhI) and recombinant human proinsulin (rhP) by free-solution capillary zone electrophoresis in uncoated fused-silica capillaries withContinue Reading
    Recombinant human proinsulin - protein A domain fusion protein (rPFP) was immobilized on synthetic matrix, resulting in inexpensive immunoglobulin-binding sorbent. This is an alternate to a protein AContinue Reading
    One of the stages in producing recombinant human insulin is the enzymatic decomposition of proinsulin by trypsin and carboxypeptidase B, insulin and C-peptide forming in almost 100% yield. InContinue Reading