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pH and protease control of acrosomal content stasis and release during the guinea pig sperm acrosome reaction.
The purpose of this study was to examine how trypsin inhibitors affect the guinea pig sperm acrosome reaction in vitro. Using spermatozoa pretreated with lysophosphatidyl choline, we found that both… Expand
Purification and characterization of the primary acrosomal autoantigen of guinea pig epididymal spermatozoa.
- D. Hardy, T. Huang, W. Driscoll, K. Tung, G. Wild
- Biology, Medicine
- Biology of reproduction
- 1 March 1988
Previous studies showed that sperm auto- and alloantigens participate in guinea pig (GP) fertilization. In an effort to determine how alloantibodies to GP sperm acrosomal contents (AC) inhibit… Expand
Purification, new assay, and properties of coenzyme A transferase from Peptostreptococcus elsdenii.
Coenzyme A (CoA) transferase from Peptostreptococcus elsdenii has been purified and crystallized, and some of its properties have been established. The work was facilitated by a newly developed… Expand
Evidence for a buried location of nigeran in the cell wall of Aspergillus niger.
An enzymic method for the large-scale preparation of nigerose and nigeran oligosaccharides.
Abstract A convenient enzymic method for the large-scale preparation of nigerose (O-α- d -glucopyranosyl-(1 → 3)- d -glucose) and a series of nigeran oligosaccharides (alternating α-(1 → 3) and α-(1… Expand
Structure of the tetrasaccharide produced by the hydrolysis of nigeran by the enzyme mycodextranase.
Determination of the action patterns of glycanases.
Abstract A simple, sensitive, rapid technique for determining the action pattern of glycanases is presented that is based on a steady-state measurement of the average chain length of the reaction… Expand
Biosynthesis of a mycobacterial lipopolysaccharide. Properties of the polysaccharide: acyl coenzyme A acyltransferase reaction.
Abstract A particulate enzyme preparation was obtained from Mycobacterium phlei cells which had the activity of a polysaccharide:acyl coenzyme A acyltransferse. We conclude that the enzyme system is… Expand
Enzymes that hydrolyze fungal cell wall polysaccharides. II. Purification and properties of mycodextranase, an endo- -D-(1 leads to 4) glucanase from Penicillium melinii.
- K. Tung, A. Rosenthal, J. Nordin
- Chemistry, Medicine
- The Journal of biological chemistry
- 25 November 1971
Abstract A specific fungal α-(1 → 4)-glucanase, mycodextranase, useful for the large scale production of nigerose, has been purified to homogeneity in high yield from the culture filtrate of… Expand