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Bacterial cell division protein FtsZ assembles into protofilament sheets and minirings, structural homologs of tubulin polymers.
The similarity of polymers formed by FtsZ and tubulin implies that the protofilament sheet is an ancient cytoskeletal system, originally functioning in bacterial cell division and later modified to make microtubules. Expand
Distribution and three-dimensional structure of AIDS virus envelope spikes
Reconciling available atomic structures with the three-dimensional whole spike density map yields insights into the orientation of Env spike structural elements and possible structural bases of their functions. Expand
Three-dimensional image reconstruction of dephosphorylated smooth muscle heavy meromyosin reveals asymmetry in the interaction between myosin heads and placement of subfragment 2
Two-dimensional crystalline arrays of the unphosphorylated inactive form of smooth muscle heavy meromyosin suitable for structural studies by electron cryomicroscopy of unstained, frozen-hydrated specimens are obtained and reveal an asymmetric interaction between the two myosin heads. Expand
Structural Organization of the Actin Cytoskeleton at Sites of Clathrin-Mediated Endocytosis
The data suggest that the primary role of the actin cytoskeleton in CME is to constrict and elongate the bud neck and drive the endocytosed vesicles from the plasma membrane. Expand
Recreation of the terminal events in physiological integrin activation
In vitro analysis confirms talin binding is sufficient for activation and extension of membrane-embedded integrin.
Electron tomography analysis of envelope glycoprotein trimers on HIV and simian immunodeficiency virus virions
  • P. Zhu, E. Chertova, +5 authors K. Roux
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences…
  • 10 December 2003
The results directly demonstrate the presence of env trimers on the surface of AIDS virus virions, albeit at numbers much lower than generally appreciated, and have important implications for understanding virion formation, virus interactions with host cells, and virus neutralization. Expand
Refined model of the 10S conformation of smooth muscle myosin by cryo-electron microscopy 3D image reconstruction.
The 10S reconstruction of thesmHMM shows additional alpha-helical coiled-coil not seen in the earlier smHMM reconstruction, but the location of one segment of S2 is the same in both, which suggests that this interaction is not simply due to crystallographic packing but is enforced by elements of the myosin heads. Expand
Tomographic 3D Reconstruction of Quick-Frozen, Ca2+-Activated Contracting Insect Flight Muscle
Motor actions of myosin were directly visualized by electron tomography of insect flight muscle quick-frozen during contraction, suggesting the MD tilts and slews on actin from weak to strong binding, followed by swinging of the LCD through an approximately 35 degrees axial angle. Expand
Structure of myosin filaments from relaxed Lethocerus flight muscle by cryo-EM at 6 Å resolution
A cryo–electron microscopy three-dimensional image reconstruction of relaxed myosin II–containing thick filaments from the flight muscle of the giant water bug Lethocerus indicus is described, revealing unique molecular motor conformation and unprecedented details of the filament backbone. Expand
Accurate marker-free alignment with simultaneous geometry determination and reconstruction of tilt series in electron tomography.
Model calculations show that it is essential to refine the geometrical parameters as well as the accurate alignment of the images to obtain a faithful map of the original structure. Expand