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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Expand
Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
A modified protocol for the generation of iPS cells that does not require the Myc retrovirus is described and, with this protocol, significantly fewer non-iPS background cells are obtained, and theiPS cells generated were consistently of high quality. Expand
Induction of Pluripotent Stem Cells From Adult Human Fibroblasts by Defined Factors
This work generated induced pluripotent stem cells capable of germline transmission from murine somatic cells by transd, and demonstrated the ability of these cells to reprogram into patient-specific and disease-specific stem cells. Expand
A more efficient method to generate integration-free human iPS cells
A simple method is reported, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors, which may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future. Expand
Anatomy of zebrafish cerebellum and screen for mutations affecting its development.
The cell types and neural tracts of the adult zebrafish cerebellum are described using molecular markers and transgenic lines and it is found that the cerebellar-like structures in the optic tectum and antero-dorsal hindbrain show similar Parvalbumin7 and Vglut1 expression profiles as the Cerebellum. Expand
Stable integration and conditional expression of electroporated transgenes in chicken embryos.
A transposon-mediated gene transfer is developed, a novel technique in chicken manipulations that facilitates an integration of a transgene into the genome when co-acting with Tol2 transposase and could be experimentally induced upon tetracycline administration at relatively late stages such as E6, where a variety of organogenesis are underway. Expand
Variation in the safety of induced pluripotent stem cell lines
This study evaluated the teratoma-forming propensity of secondary neurospheres generated from 36 mouse induced pluripotent stem (iPS) cell lines derived in 11 different ways, which correlated with the persistence of undifferentiated cells. Expand
Proneural gene-linked neurogenesis in zebrafish cerebellum.
The data indicate that proneural gene-linked neurogenesis is evolutionarily conserved in the cerebellum among vertebrates, and that the continuously generated neurons help remodel neural circuits in the adult zebrafish cerebellums. Expand
The let-7/LIN-41 pathway regulates reprogramming to human induced pluripotent stem cells by controlling expression of prodifferentiation genes.
- Kathleen A. Worringer, T. A. Rand, +6 authors S. Yamanaka
- Biology, Medicine
- Cell stem cell
- 2 January 2014
It is shown that the let-7 family of microRNAs acts as an inhibitory influence on the reprogramming process through a regulatory pathway involving prodifferentiation factors, including EGR1. Expand
Maturation, not initiation, is the major roadblock during reprogramming toward pluripotency from human fibroblasts
- K. Tanabe, Michiko Nakamura, Megumi Narita, Kazutoshi Takahashi, S. Yamanaka
- Biology, Medicine
- Proceedings of the National Academy of Sciences
- 28 June 2013
It is demonstrated that maturation, and not initiation, is the limiting step during the direct reprogramming of human fibroblasts toward pluripotency and that each proreprogramming factor has a different mode of action. Expand