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Detection of Salmonella enterica in Naturally Contaminated Liquid Eggs by Loop-Mediated Isothermal Amplification, and Characterization of Salmonella Isolates
TLDR
The LAMP assay is rapid, specific, and sensitive for Salmonella detection in liquid eggs and is able to monitorSalmonella contamination in egg-handling plants more reliably. Expand
Contamination level and ingestion dose of foodborne pathogens associated with infections
TLDR
An estimation of the infectious dose of the pathogens was obtained by conducting microbiological risk assessments in 17 outbreaks of Salmonella, E. coli, Vibrio parahaemolyticus, and Campylobacter jejuni occurring in Japan between 2004 and 2006. Expand
Rapid detection of enterotoxigenic Staphylococcus aureus harbouring genes for four classical enterotoxins, SEA, SEB, SEC and SED, by loop‐mediated isothermal amplification assay
TLDR
A loop‐mediated isothermal amplification (LAMP) assay targeting the genes for the four classical enterotoxins, SEA, SEB, SEC and SED, in Staphylococcus aureus is developed. Expand
Sensitive and rapid detection of Vero toxin-producing Escherichia coli using loop-mediated isothermal amplification.
TLDR
Results indicate that the proposed LAMP assay is a rapid, specific and sensitive method of detecting the VT-producing E. coli in food because of the difficulty of detection in food samples. Expand
Detection of Verotoxigenic Escherichia coli O157 and O26 in food by plating methods and LAMP method: a collaborative study.
TLDR
LAMP assay is effective as a screening assay to detect E. coli O157 and O26 from positive samples and it was demonstrated the LAMP assay was superior to the IMS-plating method. Expand
Migration of formaldehyde and acetaldehyde into mineral water in polyethylene terephthalate (PET) bottles
TLDR
It is suggested that in this case both FA and AA migrated from PET materials, but were subsequently decomposed by the heterotrophic bacteria in the unsterilized water. Expand
Effect of Temperature and Water Activity on the Growth of Cladosporium sphaerospermum and Cladosporium cladosporioides
TLDR
C. sphaerospermum showed a broader range of growth temperature and Aw than C. cladosporioides, and showed morphological changes such as dark brown hyphae with thick and cigar shaped cells,.and irregulary swollen cells at low Aw. Expand
Direct colony PCR of several medically important fungi using Ampdirect plus.
TLDR
All of the 63 fungal strains examined, including those of the genera Candida and Aspergillus, were amenable to DNA amplification using an Ampdirect(R) Plus kit, which allows direct PCR amplification with no requirement for DNA extraction, following 1 h of rapid fungal lysis. Expand
Surveillance of Shiga toxin-producing Escherichia coli in beef with effective procedures, independent of serotype.
TLDR
It was demonstrated that the procedure was effective for detecting STEC independent of serotype, and a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay targeting the Shiga toxin (ST) gene was established. Expand
Nivalenol and the targeting of the female reproductive system as well as haematopoietic and immune systems in rats after 90-day exposure through the diet
TLDR
In summary, NIV targets the female reproductive system as well as haematopoietic and immune systems in rats fed NIV for 90 days, and the lowest observable effect level was calculated as 0.4 mg kg−1 body weight day−1. Expand
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