Bending the MDCK Cell Primary Cilium Increases Intracellular Calcium
- H. Praetorius, K. Spring
- BiologyJournal of Membrane Biology
- 1 November 2001
The hypothesis that the primary cilium of renal epithelia is mechanically sensitive and serves as a flow sensor in MDCK cells is tested using differential interference contrast and fluorescence microscopy and concludes that it responds to flow by greatly increasing intracellular calcium.
Video Microscopy: The Fundamentals
Why Video? Microscope Image Formation. Practical Aspects of Microscopy. Physiological Characteristics of the Eye. Video Signal Fundamentals. Detection of Light and Vidicon Tube Cameras-Monochrome.…
Routes and mechanism of fluid transport by epithelia.
- K. Spring
- BiologyAnnual Review of Physiology
- 1998
It is shown that local osmosis best accounts for water movement and it seems virtually certain that the tight junctions are water permeable, the fraction of absorbed fluid that crosses the tight junction cannot yet be determined with confidence.
Removal of the MDCK Cell Primary Cilium Abolishes Flow Sensing
- H. Praetorius, K. Spring
- Biology, MedicineJournal of Membrane Biology
- 2003
The hypothesis that cell primary cilium is solely responsible for the flow-induced Ca2+ response in MDCK cells was tested by removal of the cilia from mature, responsive cells and it is estimated that a single mechanically sensitive channel in the cilum could provide the requisite Ca2-induced influx.
Low Chloride Stimulation of Prostaglandin E2Release and Cyclooxygenase-2 Expression in a Mouse Macula Densa Cell Line*
- Tianxin Yang, John M. Park, J. Schnermann
- BiologyJournal of Biological Chemistry
- 1 December 2000
It is concluded that low chloride stimulates PGE2 release and COX-2 expression in MMDD1 cells through activation of MAP kinases.
A physiological view of the primary cilium.
- H. Praetorius, K. Spring
- Biology, MedicineAnnual Review of Physiology
- 2005
This review provides an update of the current understanding about the function of primary cilia and focuses on the various functions of the primary cilium rather than on its role in pathology.
Gallbladder epithelial cell hydraulic water permeability and volume regulation
- B. Persson, K. Spring
- BiologyThe Journal of General Physiology
- 1 March 1982
Cell volume was calculated from computer reconstruction of light microscopic images of epithelial cells obtained by the "optical slice" technique and volume regulatory response was not influenced by serosal ouabain or reduction of bath NaCl concentration to 10 mM.
Size and shape of the lateral intercellular spaces in a living epithelium.
The lateral intercellular spaces of Necturus gallbladder epithelium were seen and measured while the living tissue was perfused in a new chamber. The compliance of the lateral cell membranes was…
The renal cell primary cilium functions as a flow sensor.
- H. Praetorius, K. Spring
- Biology, MedicineCurrent opinion in nephrology and hypertension
- 1 September 2003
The primary cilium functions as a flow sensor in cultured renal epithelial cells mediating a large increase in intracellular calcium concentration and to involve the proteins polycystin 1 and 2, defects in which are associated with the majority of cases of human polycystic kidney disease.
Involvement of calcium and cytoskeleton in gallbladder epithelial cell volume regulation.
- J. Foskett, K. Spring
- BiologyAmerican Journal of Physiology
- 1985
Results indicate that RVD depends on calcium, calmodulin, and an intact microfilament network, whereas RVI is independent of these factors.
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