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A Cold-Adapted Lipase of an Alaskan Psychrotroph,Pseudomonas sp. Strain B11-1: Gene Cloning and Enzyme Purification and Characterization
TLDR
The enzyme showed a 1,3-positional specificity toward triolein and was stable between pH 6 and 9, and the optimal pH for the enzymatic hydrolysis of tributyrin was around 8.2, while the enzyme was unstable at temperatures higher than 45°C.
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Selenocysteine.
TLDR
The mechanistic roles of selenocysteine residues in the glutathione peroxidase family of enzymes, the 5' deiodinases, formate dehydrogenases, glycine reductase, and a few hydrogenases are discussed.
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A new antitumor enzyme, L-lysine alpha-oxidase from Trichoderma viride. Purification and enzymological properties.
TLDR
L-Lysine alpha-oxidase from Trichoderma viride Y244-2 has been purified to homogeneity and several lysine analogs such as delta-hydroxylysine are oxidized efficiently.
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Cysteine sulfinate desulfinase, a NIFS-like protein of Escherichia coli with selenocysteine lyase and cysteine desulfurase activities. Gene cloning, purification, and characterization of a novel…
TLDR
The Escherichia coli genome contains three genes with sequence homology to nifS, and a new enzyme catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L- Cysteine sulfinate desulfinase, and L-selenocystine to produce L-alanine is named.
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Selenocysteine lyase, a novel enzyme that specifically acts on selenocysteine. Mammalian distribution and purification and properties of pig liver enzyme.
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Purification and characterization of thermostable and nonthermostable 2-haloacid dehalogenases with different stereospecificities from Pseudomonas sp. strain YL
Two novel hydrolytic dehalogenases, thermostable L-2-haloacid dehalogenase (L-DEX) inducibly synthesized by 2-chloropropionate (2-CPA) and nonthermostable DL-2-haloacid dehalogenase (DL-DEX) induced…
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Crystal structure of L-2-haloacid dehalogenase from Pseudomonas sp. YL. An alpha/beta hydrolase structure that is different from the alpha/beta hydrolase fold.
TLDR
The crystal structure of the homodimeric enzyme from Pseudomonas sp.
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Synthesis of optically active amino acids from alpha-keto acids with Escherichia coli cells expressing heterologous genes
TLDR
A simple method for enzymatic synthesis of L and D amino acids from alpha-keto acids with Escherichia coli cells which express heterologous genes is described and optic pure D enantiomers of glutamate and leucine were obtained.
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Cloning of formate dehydrogenase gene from a methanol-utilizing bacterium Mycobacterium vaccae N10
The gene of NAD+-dependent formate dehydrogenase (FDH) from Mycobacterium vaccae N10 was cloned into Escherichia coli by hybridization with digoxigenin-labeled DNA probes, which were prepared by…
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Properties of L-methionine gamma-lyase from Pseudomonas ovalis.
TLDR
The distribution of bacterial L-Methionine gamma-lyase was investigated, and Pseudomonas ovalis was found to have the highest activity of enzyme, which was inducibly formed by addition of L-methionines to the medium.
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