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Fidelity of DNA Polymerase ε Holoenzyme from Budding YeastSaccharomyces cerevisiae *
- K. Shimizu, K. Hashimoto, +4 authors A. Sugino
- The Journal of Biological Chemistry
- 4 October 2002
DNA polymerases δ and ε (pol δ and ε) are the major replicative polymerases and possess 3′-5′ proofreading exonuclease activities that correct errors arising during DNA replication in the yeast… Expand
The fifth essential DNA polymerase φ in Saccharomyces cerevisiae is localized to the nucleolus and plays an important role in synthesis of rRNA
- K. Shimizu, Y. Kawasaki, Shin-ichiro Hiraga, M. Tawaramoto, N. Nakashima, A. Sugino
- Biology, Medicine
- Proceedings of the National Academy of Sciences…
- 1 July 2002
We report that POL5 encodes the fifth essential DNA polymerase in Saccharomyces cerevisiae. Pol5p was identified and purified from yeast cell extracts and is an aphidicolin-sensitive DNA polymerase… Expand
Specificity of mutations induced by carbon ions in budding yeast Saccharomyces cerevisiae.
- Y. Matuo, S. Nishijima, Y. Hase, A. Sakamoto, A. Tanaka, K. Shimizu
- Biology, Medicine
- Mutation research
- 1 December 2006
To investigate the nature of mutations induced by accelerated ions in eukaryotic cells, the effects of carbon-ion irradiation were compared with those of gamma-ray irradiation in the budding yeast… Expand
Orientation of paramecium swimming in a DC magnetic field.
We found that a ciliated protozoan, Paramecium, swam perpendicular to a static (DC) magnetic field (0.68 T). The swimming orientation was similar even when the ionic current through the cell membrane… Expand
Fidelity of DNA polymerase epsilon holoenzyme from budding yeast Saccharomyces cerevisiae.
DNA polymerases delta and epsilon (pol delta and epsilon) are the major replicative polymerases and possess 3'-5' proofreading exonuclease activities that correct errors arising during DNA… Expand
AtREV1, a Y-Family DNA Polymerase in Arabidopsis, Has Deoxynucleotidyl Transferase Activity in Vitro1[W]
To clarify the functions of the Arabidopsis thaliana REV1 (AtREV1) protein, we expressed it in Escherichia coli and purified it to near homogeneity. The deoxynucleotidyl transferase activity of the… Expand
Comparison of rapid and simple colorimetric microplate assays as an index of bacterial count
Abstract We investigated rapid and simple colorimetric microplate assays with tryptone–yeast–glucose broth (TYG) containing oxidation–reduction (redox) indicators (alamarBlue, MTS and WST-8),… Expand
Biological effects of carbon ion beams with various LETs on budding yeast Saccharomyces cerevisiae.
It has been established that irradiation with higher linear energy transfer (LET) increases lethality and mutagenicity more than that with lower LET. However, the characteristics specific to carbon… Expand
Evaluation of DNA lesions caused by high-LET radiation using the polymerase chain reaction
Abstract Generally, it is thought that the bio-effect by the radiation depends on LET. Compared to low-LET radiation, High-LET radiation induces a large number of single and double stranded breaks.… Expand
Fidelity of DNA polymerase delta holoenzyme from Saccharomyces cerevisiae: the sliding clamp proliferating cell nuclear antigen decreases its fidelity.
DNA polymerases delta and epsilon (pol delta and epsilon) are the two major replicative polymerases in the budding yeast Saccharomyces cerevisiae. The fidelity of pol delta is influenced by its 3'-5'… Expand