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C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector
TLDR
LshC2c2 is a RNA-guided RNase which requires the activity of its two HEPN domains, suggesting previously unidentified mechanisms of RNA targeting and degradation by CRISPR systems.
Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system.
TLDR
It is shown that matching sequences that are no longer able to elicit defense, still guide the CRISPR/Cas acquisition machinery to foreign DNA, thus making the spacer acquisition process adaptive and leading to restoration of CRISpr/Cas-mediated protection.
Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence
TLDR
It is proposed that the crRNA seed sequence plays a role in the initial scanning of invader DNA for a match, before base pairing of the full-length spacer occurs, which may enhance the protospacer locating efficiency of the E. coli Cascade complex.
Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature.
This review presents recommended nomenclature for the biosynthesis of ribosomally synthesized and post-translationally modified peptides (RiPPs), a rapidly growing class of natural products. The
A third recognition element in bacterial promoters: DNA binding by the alpha subunit of RNA polymerase.
TLDR
Results suggest that UP elements comprise a third promoter recognition region (in addition to the -10, -35 recognition hexamers, which interact with the sigma subunit) and may account for the presence of (A+T)-rich DNA upstream of many prokaryotic promoters.
Diversity and evolution of class 2 CRISPR–Cas systems
TLDR
A comprehensive census of class 2 types and class 2 subtypes in complete and draft bacterial and archaeal genomes is presented, evolutionary scenarios for the independent origin of different class 2 CRISPR–Cas systems from mobile genetic elements are outlined, and an amended classification and nomenclature of CRISpr–Cas is proposed.
Escherichia coli CspA-family RNA chaperones are transcription antiterminators.
TLDR
CspA, as well as homologous RNA chaperones CspE and CspC, are transcription antiterminators, which is mediated by CspA and other cold shock-induced Csp proteins.
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