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Gel-Based and Gel-Free Quantitative Proteomics Approaches at a Glance
TLDR
The goal of this perspective assay is to present the balance between the strengths and weaknesses of the available gel-based and -free methods and their application to plants. Expand
Prolyl oligopeptidase stimulates the aggregation of α-synuclein
TLDR
It is found that in vitro incubation of the protein with PO did not result in truncation of full-length alpha-synuclein, and an acceleration of the aggregation process of alpha- synuclein using turbidity measurements that was reversed by specific inhibitors of PO enzymatic activity. Expand
Proteome analysis of non-model plants: a challenging but powerful approach.
TLDR
The power of 2DE for non-model plants in combination with multivariate data analysis and MS/MS identification and will evaluate possible alternatives are illustrated. Expand
Copper Trafficking in Plants and Its Implication on Cell Wall Dynamics
TLDR
In the current review an overview is presented on the different stages of Cu transport from the soil into the plant and throughout the different plant tissues, and the involvement of numerous Cu-proteins and Cu-dependent activities in the properties of one of the major Cu-accumulation sites in plants: the cell wall. Expand
Acute metal stress in Populus tremula×P. alba (717‐1B4 genotype): Leaf and cambial proteome changes induced by cadmium2+
TLDR
An important tissue specificity of the response was observed, according to the differences in cell structures and functions of Populus tremula×P. Expand
Identification of a gene coding for a deglycosylating enzyme in Hypocrea jecorina.
TLDR
An enzyme with mannosyl glycoprotein endo-N-acetyl-beta-D-glucosaminidase (ENGase)-type activity was partially purified from the extracellular medium of the mould Hypocrea jecorina and used to identify the gene in the T. reesei genome. Expand
Target or barrier? The cell wall of early- and later-diverging plants vs cadmium toxicity: differences in the response mechanisms
TLDR
The present review analyzes the dual property of plant cell walls, i.e., barrier and target of heavy metals, by taking Cd toxicity as example, and compares the wall-related mechanisms in early- and later-diverging land plants, by considering the diversity in cell wall composition. Expand
Activation, Proteolytic Processing, and Peptide Specificity of Recombinant Cardosin A*
TLDR
The primary specificity of the enzyme closely resembles those of cathepsin D and plasmepsins, suggesting that cardosin A shares the same peptide scissile bond preferences of its vacuolar/lysosomal mammalian and protozoan homologues. Expand
A case study of de novo sequence analysis of N-sulfonated peptides by MALDI TOF/TOF mass spectrometry
TLDR
It is shown that full-length de novo sequencing is possible using MALDI TOF/TOF analysis, and the influence of the presence of internal Pro, acidic and basic residues on the TOF-TOF fragmentation pattern will be discussed, both for underivatized and guanidinated/sulfonated peptides. Expand
One dry summer: a leaf proteome study on the response of oak to drought exposure.
TLDR
The data obtained indicated that the oak tried to adapt its metabolism in order to maintain its full molecular functionality, but prolonged drought exposure overwhelmed the adaptive mechanisms and at the last sampling point of this study the molecular machinery succumbed. Expand
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