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Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character
Two linear deoxyribonucleic acid plasmids were isolated from the yeast Kluyveromyces lactis IFO 1267 and a single chromosomal gene was found which was responsible for the resistance to the K. lactis killer.
Invertrons, a class of structurally and functionally related genetic elements that includes linear DNA plasmids, transposable elements, and genomes of adeno-type viruses.
- K. Sakaguchi
- Biology, MedicineMicrobiological reviews
- 1 March 1990
A model for replication and integration of invertrons is presented, as well as a model for transposition of transposable elements.
Xylose (glucose) isomerase gene from the thermophile Thermus thermophilus: cloning, sequencing, and comparison with other thermostable xylose isomerases
Analysis of amino acid substitutions that distinguish the Thermus xylose isomerase from other thermostable xylOSE isomerases suggests that the further increase in thermostability in T. thermophilus is due to substitution of amino acids which react during irreversible inactivation and results also from increased hydrophobicity.
Fusion of protoplasts and genetic recombination of Brevibacterium flavum.
Protoplasts of Brevibacterium flavum cells were obtained by treatment with 0.3 units of penicillin per ml during exponential growth, and followed by lysozyme treatment in a hypertonic medium.…
Analysis of mutations in cyclodextrin glucanotransferase from Bacillus stearothermophilus which affect cyclization characteristics and thermostability
- S. Fujiwara, H. Kakihara, K. Sakaguchi, T. Imanaka
- Medicine, BiologyJournal of bacteriology
- 1 November 1992
Cyclodextrin glucanotransferase (CGTase; EC 220.127.116.11) produces cyclodextrin from starch. The CGTase molecule is composed of four globular domains, A, B, C, and D. In order to gain better…
Cyclization characteristics of cyclodextrin glucanotransferase are conferred by the NH2-terminal region of the enzyme
- S. Fujiwara, H. Kakihara, +4 authors T. Imanaka
- Medicine, BiologyApplied and environmental microbiology
- 1 December 1992
It was found that the cyclization reaction was conferred by the NH2-terminal region of CGTase and that the thermostability of some chimeric enzymes was lower than that of the parental CGTases.
A LINEAR PLASMID-LIKE DNA IN STREPTOMYCES SP. PRODUCING LANKACIDIN GROUP ANTIBIOTICS
Results suggest that the linear plasmid-like DNA was isolated from a lysate of Streptomyces sp.
A linear DNA plasmid from Streptomyces rochei with an inverted terminal repetition of 614 base pairs.
An inverted terminal repetition of 614 bp was found along with the presence of further interrupted homologous sequences beyond this area up to 800 bp, which are the first inverted terminal repeat sequences found in microbial linear plasmids.
A spectrophotometric assay for the cyclization activity of cyclomaltohexaose (alpha-cyclodextrin) glucanotransferase.
A cyclomaltohexaose (alpha-cyclodextrin) determination method which is both highly reproducible and selective is described and should be versatile in assaying diverse cyclomal to hexaose glucanotransferase enzymes.
Comparative studies onChlorella cell walls: Induction of protoplast formation
Among 12 strains of Chlorella ellipsoidea, C. vulgaris, and C. saccharophila tested, 4 strains formed osmotically labile protoplasts after treatment with mixtures of polysaccharide degrading enzymes, indicating the chemical composition of the cell wall would be somewhat different from that of other types.