• Publications
  • Influence
Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs
Human and mouse embryonic stem cells (ESCs) are derived from blastocyst-stage embryos but have very different biological properties, and molecular analyses suggest that the pluripotent state of humanExpand
  • 751
  • 73
  • PDF
Direct cell reprogramming is a stochastic process amenable to acceleration
Direct reprogramming of somatic cells into induced pluripotent stem (iPS) cells can be achieved by overexpression of Oct4, Sox2, Klf4 and c-Myc transcription factors, but only a minority of donorExpand
  • 942
  • 55
  • PDF
Pluripotency and Cellular Reprogramming: Facts, Hypotheses, Unresolved Issues
Direct reprogramming of somatic cells to induced pluripotent stem cells by ectopic expression of defined transcription factors has raised fundamental questions regarding the epigenetic stability ofExpand
  • 646
  • 33
  • PDF
Combinatorial Development of Biomaterials for Clonal Growth of Human Pluripotent Stem Cells
Both human embryonic stem (hES) cells and induced pluripotent stem (hiPS) cells can self-renew indefinitely in culture, however current methods to clonally grow them are inefficient andExpand
  • 448
  • 12
  • PDF
Technical challenges in using human induced pluripotent stem cells to model disease.
Reprogramming of human somatic cells uses readily accessible tissue, such as skin or blood, to generate embryonic-like induced pluripotent stem cells (iPSCs). This procedure has been applied toExpand
  • 369
  • 9
  • PDF
Crispr democracy: Gene editing and the need for inclusive deliberation
Not since the early, heady days of recombinant DNA (rDNA) has a technique of molecular biology so gripped the scientific imagination as the CRISPR-Cas9 method of gene editing. Its promises areExpand
  • 80
  • 4
  • PDF
Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing
Writing specific DNA sequences into the human genome is challenging with non-viral gene-editing reagents, since most of the edited sequences contain various imprecise insertions or deletions. WeExpand
  • 55
  • 3
Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions
The current gold standard for the culture of human pluripotent stem cells requires the use of a feeder layer of cells. Here, we develop a spatially defined culture system based on UV/ozone radiationExpand
  • 132
  • 2
  • PDF
Establishment of Reporter Lines for Detecting Fragile X Mental Retardation (FMR1) Gene Reactivation in Human Neural Cells
Human patient‐derived induced pluripotent stem cells (hiPSCs) provide unique opportunities for disease modeling and drug development. However, adapting hiPSCs or their differentiated progenies toExpand
  • 32
  • 2
Research ethics: Treat donors as partners in biobank research
Proposed rules to protect research subjects will impede progress, say Krishanu Saha and J. Benjamin Hurlbut. Instead, give donors more say in how samples are used.
  • 42
  • 2