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Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains.
TLDR
Heterodimeric Fab fragments can be assembled on the surface of the phages by linking one chain to the phage coat protein, and secreting the other into the bacterial periplasm by introducing an amber mutation. Expand
Sequence and expression of a major egg antigen from Schistosoma mansoni. Homologies to heat shock proteins and alpha-crystallins.
TLDR
This work isolated and sequenced cDNA clones encoding two variants of the p40 antigen and expressed one p40 clone in Escherichia coli and identified a fusion protein which elicits antibodies which immunoprecipitate p40 and recognise antigens of identical sizes in S. haematobium and S. bovis. Expand
Vaccination against ovine cysticercosis using a defined recombinant antigen
TLDR
The expression in Escherichia coli of complementary DNA encoding T. ovis antigens as fusion proteins with the Schistosoma japonicum glutathione S-transferase gives significant, although not complete, immunity against challenge infection with T. Ovis eggs. Expand
Phylogenetic distribution of cysteine proteinases in beetles: evidence for an evolutionary shift to an alkaline digestive strategy in Cerambycidae.
TLDR
The findings suggest that either a close correlation between midgut pH and the proteolytic pH optimum is not needed for adequate digestive efficiency, or that midGut pH is a more constrained digestive feature and there has been insufficient time for it to shift upwards to maximize serine proteinase activity. Expand
Pathfinder selection: in situ isolation of novel antibodies.
TLDR
This work demonstrates that an existing binding specificity can be used as a tool to select phage libraries in situ, obviating the need to purify or clone the target. Expand
Toxicity of Bacillus thuringiensis var. kurstaki to three nontarget Lepidoptera in field studies
TLDR
The results of these studies indicate that B. thuringiensis sprays are toxic to some nontarget lepidopterans for at least 30 d after the spray. Expand
Combinatorial infection and in vivo recombination: a strategy for making large phage antibody repertoires.
TLDR
A model system, involving the lox-Cre site-specific recombination system of bacteriophage PI, to lock together the heavy and light chain genes from two different replicons within an infected bacterium is described. Expand
Directing phage selections towards specific epitopes.
TLDR
Competitive deselection is a viable means for directing selections towards useful epitopes and permits a more effective 'search' of phage display repertoires and allows the emergence of lower affinity clones with useful specificities. Expand
A fully human antibody neutralising biologically active human TGFbeta2 for use in therapy.
TLDR
The strategy employed was to isolate a human single chain Fv fragment that neutralises human TGF beta2 from a phage display repertoire, convert it into a human IgG4 and then determine its TGFbeta binding and neutralisation properties and its physical characteristics. Expand
Bioactive neolignans from the leaves of Magnolia virginiana
Abstract A novel biphenyl ether, 4,4′-diallyl-2,3′-dihydroxybiphenyl ether, 3,5′-diallyl-2′-hydroxy-4-methoxy-biphenyl and 5,5′-diallyl-2,2′-dihydroxybiphenyl (magnolol) were isolated from the leavesExpand
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