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Environmental regulation of lateral root initiation in Arabidopsis.
Plant morphology is dramatically influenced by environmental signals. The growth and development of the root system is an excellent example of this developmental plasticity. Both the number andExpand
Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A
Significance Microbes have the genetic capacity to produce large numbers of specialized compounds, yet produce only a small fraction of these in the laboratory. Here we introduce a genetic platformExpand
Crystallographic trapping in the rebeccamycin biosynthetic enzyme RebC
The biosynthesis of rebeccamycin, an antitumor compound, involves the remarkable eight-electron oxidation of chlorinated chromopyrrolic acid. Although one rebeccamycin biosynthetic enzyme is capableExpand
Divergent pathways in the biosynthesis of bisindole natural products.
Two molecules of the amino acid L-tryptophan are the biosynthetic precursors to a class of natural products named the "bisindoles." Hundreds of these bisindole molecules have been isolated fromExpand
Metal Binding Properties of Escherichia coli YjiA, a Member of the Metal Homeostasis-Associated COG0523 Family of GTPases
GTPases are critical molecular switches involved in a wide range of biological functions. Recent phylogenetic and genomic analyses of the large, mostly uncharacterized COG0523 subfamily of GTPasesExpand
The FAD Cofactor of RebC Shifts to an IN Conformation upon Flavin Reduction†,‡
RebC is a putative flavin hydroxylase functioning together with RebP to carry out a key step in the biosynthesis of rebeccamycin. To probe the mechanism of flavin-based chemistry in RebC, we solvedExpand
Introducing the parvome: bioactive compounds in the microbial world.
We describe and discuss the features and functions of the "parvome", the "-ome" of the chemical world, consisting of the small molecules produced by living organisms. Here, we focus specifically onExpand
The Violacein Biosynthetic Enzyme VioE Shares a Fold with Lipoprotein Transporter Proteins*
VioE, an unusual enzyme with no characterized homologues, plays a key role in the biosynthesis of violacein, a purple pigment with antibacterial and cytotoxic properties. Without bound cofactors orExpand
A heme-dependent enzyme forms the nitrogen-nitrogen bond in piperazate.
Molecules containing a nitrogen-nitrogen (N-N) linkage have a variety of structures and biological activities; however, no enzyme has yet been demonstrated to catalyze N-N bond formation in anExpand
Piperazic acid-containing natural products: structures and biosynthesis.
Covering: up to the end of 2018Piperazic acid is a cyclic hydrazine and a non-proteinogenic amino acid found in diverse non-ribosomal peptide (NRP) and hybrid NRP-polyketide (PK) structures.Expand