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Distribution and three-dimensional structure of AIDS virus envelope spikes
Reconciling available atomic structures with the three-dimensional whole spike density map yields insights into the orientation of Env spike structural elements and possible structural bases of their functions. Expand
Antibody Domain Exchange Is an Immunological Solution to Carbohydrate Cluster Recognition
The extraordinary configuration of this antibody provides an extended surface, with newly described binding sites, for multivalent interaction with a conserved cluster of oligomannose type sugars on the surface of gp120, finding a previously unappreciated mechanism for high-affinity recognition of carbohydrate or other repeating epitopes on cell or microbial surfaces. Expand
An improved smaller biotin ligase for BioID proximity labeling
A smaller promiscuous biotin ligase for proximity biotinylation called BioID2 enables more-selective targeting of fusion proteins, requires less biotin supplementation, exhibits enhanced labeling ofExpand
High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR.
The characteristics of TD PCR are investigated that serve to minimize the need to optimize annealing temperature or buffer conditions and yet produce single strong target amplicons and it is demonstrated that priming initiates above the optimum annealed temperature. Expand
Electron tomography analysis of envelope glycoprotein trimers on HIV and simian immunodeficiency virus virions
  • P. Zhu, E. Chertova, +5 authors K. Roux
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences…
  • 10 December 2003
The results directly demonstrate the presence of env trimers on the surface of AIDS virus virions, albeit at numbers much lower than generally appreciated, and have important implications for understanding virion formation, virus interactions with host cells, and virus neutralization. Expand
Effects of roasting, blanching, autoclaving, and microwave heating on antigenicity of almond (Prunus dulcis L.) proteins.
ELISA assays and Western blotting experiments that used both rabbit polyclonal antibodies and human IgE from pooled sera indicated antigenic stability of almond proteins when compared with that of the unprocessed counterpart. Expand
Optimization and troubleshooting in PCR.
  • K. Roux
  • Biology, Medicine
  • PCR methods and applications
  • 1 April 1995
Various optimization strategies are discussed, including touchdown PCR and hot-start PCR, which aims to vary one or more of the many parameters that are known to contribute to primer-template fidelity and primer extension. Expand
Detection and stability of the major almond allergen in foods.
Western blots confirm a general stability of the various peptides that comprise this complex molecule and show that the rabbit antibody-based assay recognizes substantially the same set of peptides as does the IgE in sera from almond-allergic patients. Expand
Flexibility of human IgG subclasses.
A variable region (Id)-matched set of genetically engineered human IgG1, -2, -3, and -4 subclass molecules was analyzed by electron microscopy for hinge-mediated differences in flexibility, showing the higher propensity to form closed bivalent ring Id-anti-Id dimers with IgG3 than with Igg2 and IgG4. Expand
Structure and specificity of lamprey monoclonal antibodies
The production of recombinant VLR-B antibodies specific for BclA, a major coat protein of Bacillus anthracis spores is described and it is predicted that these unusual LRR-based monoclonal antibodies will find many biomedical uses. Expand