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Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes.
CAZypedia was initiated in 2007 to create a comprehensive, living encyclopedia of the carbohydrate-active enzymes (CAZymes) and associated carbohydrate-binding modules involved in the synthesis,Expand
Time dependent inhibition of xanthine oxidase in irradiated solutions of folic acid, aminopterin and methotrexate.
A direct correlation was observed between the concentrations of the 6-formylpteridine derivatives existing in solution and the ability of these solutions to inhibit xanthine oxidase when aqueous solutions of folic acid, aminopterin and methotrexate were exposed to uv light. Expand
Remarkable Reactivity Differences between Glucosides with Identical Leaving Groups.
Insight into the source of these surprising rate differences was obtained through a comprehensive study of the nonenzymatic (spontaneous) hydrolysis of these same substrates, which revealed that ground state destabilization and transition state stabilizing effects contribute almost equally to the observed reactivity differences. Expand
Development and Application of a High-Throughput Functional Metagenomic Screen for Glycoside Phosphorylases.
A high-throughput functional screening method based on molybdenum blue formation provides a screening paradigm platform for recovering diverse GPs from uncultivated microbial communities acting on different substrates with considerable potential to unravel previously unknown degradative pathways within microbiomes. Expand
Quantification of the total neuraminidase content of recent commercially-available influenza vaccines: Introducing a neuraminidase titration reagent.
The use of a recently developed fluorometric titration reagent, TR1, is described to efficiently quantify the total enzymatically active NA content of six commercially-available influenza vaccines, including split/subunit, inactivated/live and standard /high dose products distributed from 2015/16 to 2017/18 in North America. Expand
Modulating the Nucleophile of a Glycoside Hydrolase through Site-Specific Incorporation of Fluoroglutamic Acids.
This study represents the first example of site-specific introduction of fluorinated glutamic acids into any protein and provides unique insights into the synergy of nucleophilic and acid/base catalysis within an enzyme active site. Expand
Understanding viral neuraminidase inhibition by substituted difluorosialic acids.
Measurement of rate constants for spontaneous hydrolysis of a series of substituted DFSAs reveals, surprisingly, that inherent inductive effects play very little role in this rate reduction and that interactions with the enzyme are more important. Expand