K. R. Chary

Publications121
h-index20
Citations1,305
Highly Influential Citations46
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Regulatory and structural EF-hand motifs of neuronal calcium sensor-1: Mg 2+ modulates Ca 2+ binding, Ca 2+ -induced conformational changes, and equilibrium unfolding transitions.
Neuronal calcium sensor-1 (NCS-1) is a major modulator of Ca(2+) signaling with a known role in neurotransmitter release. NCS-1 has one cryptic (EF1) and three functional (EF2, EF3, and EF4) EF-hand… Expand
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N-terminal Myristoylation Regulates Calcium-induced Conformational Changes in Neuronal Calcium Sensor-1*
Neuronal calcium sensor-1 (NCS-1), a Ca2+-binding protein, plays an important role in the modulation of neurotransmitter release and phosphatidylinositol signaling pathway. It is known that the… Expand
  • 41
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A tracked approach for automated NMR assignments in proteins (TATAPRO)
A novel automated approach for the sequence specific NMR assignments of 1HN, 13Cα, 13Cβ, 13C′/1Hα and 15N spins in proteins, using triple resonance experimental data, is presented. The algorithm,… Expand
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NMR structure of a parallel-stranded DNA duplex at atomic resolution.
DNA dodecamers have been designed with two cytosines on each end and intervening A and T stretches, such that the oligomers have fully complementary A:T base pairs when aligned in the parallel… Expand
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Mycobacterium tuberculosis H37Rv ESAT‐6–CFP‐10 complex formation confers thermodynamic and biochemical stability
The 6‐kDa early secretory antigenic target (ESAT‐6) and culture filtrate protein‐10 (CFP‐10), expressed from the region of deletion‐1 (RD1) of Mycobacterium tuberculosis H37Rv, are known to play a… Expand
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Molecular conformation of gonadoliberin using two-dimensional NMR spectroscopy.
Complete resonance assignments of the proton NMR spectrum of gonadoliberin (in its native amide and free acid forms) have been obtained using two-dimensional nuclear magnetic resonance spectroscopy… Expand
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Solution structure and calcium-binding properties of M-crystallin, a primordial betagamma-crystallin from archaea.
The lens betagamma-crystallin superfamily has many diverse but topologically related members belonging to various taxa. Based on structural topology, these proteins are considered to be… Expand
  • 47
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Selective `unlabeling' of amino acids in fractionally 13C labeled proteins: An approach for stereospecific NMR assignments of CH3 groups in Val and Leu residues
A novel methodology for stereospecific NMR assignments of methyl (CH3) groups of Val and Leu residues in fractionally 13C-labeled proteins is presented. The approach is based on selective… Expand
  • 32
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NMR characterisation of a triple stranded complex formed by homo-purine and homo-pyrimidine DNA strands at 1:1 molar ratio and acidic pH.
Homo-purine (d-TGAGGAAAGAAGGT) and homo-pyrimidine (d-CTCCTTTCTTCC) oligomers have been designed such that they are complementary in parallel orientation. When mixed in a 1:1 molar ratio, the system… Expand
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Energetics and mechanism of Ca2+ displacement by lanthanides in a calcium binding protein.
The displacement of Ca(2+) by trivalent lanthanide ions (Tm(3+)) in a protozoan (Entamoeba histolytica) Ca(2+) binding protein has been studied by NMR and isothermal calorimetry (ITC). The study… Expand
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