Superoxide generation by endothelial nitric oxide synthase: the influence of cofactors.
- J. Vasquez-Vivar, B. Kalyanaraman, K. Pritchard
- Biology, ChemistryProceedings of the National Academy of Sciences…
- 4 August 1998
Results demonstrate that superoxide is generated from the oxygenase domain by dissociation of the ferrous-dioxygen complex and that occupation of the L-arginine binding site does not inhibit this process, and indicate that modulation of BH4 concentration may regulate the ratio of superoxide to nitric oxide generated by eNOS.
Chronic exercise in dogs increases coronary vascular nitric oxide production and endothelial cell nitric oxide synthase gene expression.
This finding suggests that exercise can provide a stimulus for the enhanced production of EDRF/NO, thus possibly contributing to the beneficial effects of exercise on the cardiovascular system.
Native low-density lipoprotein increases endothelial cell nitric oxide synthase generation of superoxide anion.
To examine mechanisms by which native low-density lipoprotein (n-LDL) perturbs endothelial cell (EC) release of superoxide anion (O2-) and nitric oxide (NO), ECs were incubated with n-LDL at 240 mg…
Heat Shock Protein 90 Mediates the Balance of Nitric Oxide and Superoxide Anion from Endothelial Nitric-oxide Synthase*
Data show that Hsp 90 is essential for eNOS-dependent ·NO production and that inhibition of ATP-dependent conformational changes in Hsp90 uncouples eN OS activity and increases eNos-dependent O⨪2production.
Involvement of nitric oxide and nitrosothiols in relaxation of pulmonary arteries to peroxynitrite.
- M. Wu, K. Pritchard, P. Kaminski, R. Fayngersh, T. Hintze, M. Wolin
- Biology, ChemistryAmerican Journal of Physiology
- 1 May 1994
ONOO- appears to relax vascular tissue, in part, by nitrosylating tissue GSH (or other thiols), which subsequently releases NO over prolonged time periods.
Erythropoietin protects the infant heart against ischemia–reperfusion injury by triggering multiple signaling pathways
- P. Rafiee, Y. Shi, J. Su, K. Pritchard, J. Tweddell, J. Baker
- Medicine, BiologyBasic Research in Cardiology
- 1 May 2005
EPO increases immediate cardioprotection through the activation of multiple signal transduction pathways through theactivation of multiple signaling pathways.
Acute cardioprotective effects of erythropoietin in infant rabbits are mediated by activation of protein kinases and potassium channels
- Yang Shi, P. Rafiee, Jidong Su, K. Pritchard, J. Tweddell, J. Baker
- Biology, MedicineBasic Research in Cardiology
The rapid activation of potassium channels and protein kinases by erythropoietin represents an important new mechanism for increasing cardioprotection in infant rabbit hearts.
Endothelial nitric oxide synthase-dependent superoxide generation from adriamycin.
- J. Vasquez-Vivar, P. Martásek, N. Hogg, B. Masters, K. Pritchard, B. Kalyanaraman
- Biology, ChemistryBiochemistry
- 23 September 1997
It is demonstrated here that the endothelial isoform of nitric oxide synthase (eNOS) reduces adriamycin to the semiquinone radical, which enhances superoxide formation and Nitric oxide production and leads eNOS to generate peroxynitrite and hydrogen peroxide, potent oxidants implicated in several vascular pathologies.
Bradykinin-Induced Dilation of Human Coronary Arterioles Requires NADPH Oxidase–Derived Reactive Oxygen Species
- B. T. Larsen, A. Bubolz, Suelhem A. Mendoza, K. Pritchard, D. Gutterman
- Biology, MedicineArteriosclerosis, Thrombosis and Vascular Biology
- 1 May 2009
It is concluded that endothelial NADPH oxidase is a functionally relevant source of H2O2 that mediates agonist-induced dilation in the human heart.
Effects of D-4F on Vasodilation and Vessel Wall Thickness in Hypercholesterolemic LDL Receptor–Null and LDL Receptor/Apolipoprotein A-I Double-Knockout Mice on Western Diet
Although D-4F restores vascular endothelial cell and eNOS function to increase vasodilation, HDL containing apoA-I, or at least some critical concentration of the antiatherogenic lipoprotein, is required for D- 4F to decrease vessel wall thickness.