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(p)ppGpp: still magical?
The fundamental details of how nutritional stress leads to elevating (p)ppGpp are questionable. By common usage, the meaning of the stringent response has evolved from the specific response toExpand
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Circuitry linking the Csr and stringent response global regulatory systems.
CsrA protein regulates important cellular processes by binding to target mRNAs and altering their translation and/or stability. In Escherichia coli, CsrA binds to sRNAs, CsrB and CsrC, whichExpand
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Differential regulation by ppGpp versus pppGpp in Escherichia coli
Both ppGpp and pppGpp are thought to function collectively as second messengers for many complex cellular responses to nutritional stress throughout biology. There are few indications that theirExpand
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ppGpp is the major source of growth rate control in E. coli.
It is widely accepted that the DNA, RNA and protein content of Enterobacteriaceae is regulated as a function of exponential growth rates; macromolecular content increases with faster growthExpand
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Antagonistic Regulation of Escherichia coli Ribosomal RNA rrnB P1 Promoter Activity by GreA and DksA*
The Escherichia coli proteins DksA, GreA, and GreB are all structural homologs that bind the secondary channel of RNA polymerase (RNAP) but are thought to act at different levels of transcription.Expand
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From (p)ppGpp to (pp)pGpp: Characterization of Regulatory Effects of pGpp Synthesized by the Small Alarmone Synthetase of Enterococcus faecalis.
UNLABELLED The bacterial stringent response (SR) is a conserved stress tolerance mechanism that orchestrates physiological alterations to enhance cell survival. This response is mediated by theExpand
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Imprecise transcription termination within Escherichia coli greA leader gives rise to an array of short transcripts, GraL
We report that greA expression is driven by two strong, overlapping P1 and P2 promoters. The P1 promoter is σ70-dependent and P2 is σE-dependent. Two-thirds of transcripts terminate within the leaderExpand
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TraR, a Homolog of a RNAP Secondary Channel Interactor, Modulates Transcription
Recent structural and biochemical studies have identified a novel control mechanism of gene expression mediated through the secondary channel of RNA Polymerase (RNAP) during transcription initiation.Expand
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Effects on growth by changes of the balance between GreA, GreB, and DksA suggest mutual competition and functional redundancy in Escherichia coli.
It is well known that ppGpp and DksA interact with bacterial RNA polymerase (RNAP) to alter promoter activity. This study suggests that GreA plays a major role and GreB plays a minor role in theExpand
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Replication of oriJ-based plasmid DNA during the stringent and relaxed responses of Escherichia coli.
The oriJ-based plasmids contain the origin of DNA replication from the cryptic Rac prophage, present in the chromosomes of most Escherichia coli K-12 strains. The organization of the oriJ replicationExpand
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