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Comparison of short-term estrogenicity tests for identification of hormone-disrupting chemicals.
The aim of this study was to compare results obtained by eight different short-term assays of estrogenlike actions of chemicals conducted in 10 different laboratories in five countries. Twenty chem...
Development of an ELISA for vitellogenin in whole body homogenate of zebrafish (Danio rerio).
The yolk protein, lipovitellin (Lv) was purified from ovaries of mature female zebrafish by gel filtration and anion exchange chromatography and anti-Lv IgG was used to develop a direct non-competitive sandwich ELISA to measure Vtg concentrations of whole body homogenate (WBH) in zebra fish. Expand
Comparison of Short-Term Estrogenicity Tests for Identification of Hormone-Disrupting Chemicals
Short-term tests of estrogenlike actions of chemicals conducted in 10 different laboratories in five countries demonstrate that careful standardization is necessary to obtain a reasonable degree of reproducibility and that similar methods vary in their sensitivity to estrogenic compounds. Expand
Cadmium in the shore crab Carcinus maenas: seasonal variation in cadmium content and uptake and elimination of cadmium after administration via food.
The distribution of cadmium among tissues in crabs collected at uncontaminated sites also corresponds better with results obtained after administration of Cadmium via the food than via water, and the exposure of the crabs to cadmiam via theFood is large enough to explain the increase in concentration between May and June. Expand
In vivo comparison of xenoestrogens using rainbow trout vitellogenin induction as a screening system
Vitellogenin induction in rainbow trout after intraperitoneal injection of test compounds was investigated and the system was used as a short-term in vivo screen for estrogenicity and as a tool for establishing the priority of compounds to be tested by water exposure of fish. Expand
Estrogenicity of xenobiotics in rainbow trout (Oncorhynchus mykiss) using in vivo synthesis of vitellogenin as a biomarker
Nonylphenol and bisphenol A had the highest estrogenic potency of the xenobiotics increasing the vitellogenin level approximately 300-fold while BBP was only weakly estrogenic, increasing the concentration about 3 times. Expand
The preservatives ethyl-, propyl- and butylparaben are oestrogenic in an in vivo fish assay.
Ethylparaben was found to be approximately sixty times weaker than propyl- and butyl paraben which had oestrogenic potencies comparable to those previously found for bisphenol A. Expand
Purification and characterization of a cadmium-induced metallothionein from the shore crab Carcinus maenas (L.).
Carcinus maenas shows a high degree of structural similarity to metallothioneins from the decapod crustaceans Scylla serrata and Homarus americanus, indicating that high cadmium concentrations either inhibit theprocessing enzymes and/or that the processing enzymes cannot keep pace with the increased metallothsionein synthesis when cadmiam availability is high. Expand
Evaluation of yolk protein levels as estrogenic biomarker in bivalves; comparison of the alkali-labile phosphate method (ALP) and a species-specific immunoassay (ELISA).
Hemolymph ALP levels cannot serve as biomarker for estrogenic exposure during the pre-spawning and spawning period in U. tumidus and the utility of ALP and yolk protein levels as biomarkers in bivalves are discussed. Expand
Estrogenic effect of propylparaben (propylhydroxybenzoate) in rainbow trout Oncorhynchus mykiss after exposure via food and water.
Propylparaben showed little tendency to bioaccumulation in rainbow trout; less than 1 per thousand of the total amount of propyl paraben administered orally at 1830 mg kg(-1) 2 d (-1) over the 10-d experimental period was retained in muscle and liver 24 h after the end of the experiment. Expand