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Optimization of probe length and the number of probes per gene for optimal microarray analysis of gene expression.
Gene-specific oligonucleotide probes are currently used in microarrays to avoid cross-hybridization of highly similar sequences. We developed an approach to determine the optimal number and length of… Expand
Sensitivity issues in DNA array-based expression measurements and performance of nylon microarrays for small samples.
- F. Bertucci, K. Bernard, +6 authors B. Jordan
- Biology, Medicine
- Human molecular genetics
- 1 September 1999
DNA or oligonucleotide arrays are widely used for large-scale expression measurements, using various implementations: macroarrays in which DNA is spotted onto nylon membranes of relatively large… Expand
Acid-Induced Gene Expression in Helicobacter pylori: Study in Genomic Scale by Microarray
ABSTRACT To understand the RNA expression in response to acid stress ofHelicobacter pylori in genomic scale, a microarray membrane containing 1,534 open reading frames (ORFs) from strain 26695 was… Expand
A stable double-stranded DNA-ethidium homodimer complex: application to picogram fluorescence detection of DNA in agarose gels.
- A. Glazer, K. Peck, R. Mathies
- Chemistry, Medicine
- Proceedings of the National Academy of Sciences…
- 1 May 1990
The complex between double-stranded DNA and ethidium homodimer (5,5'-diazadecamethylene)bis(3,8-diamino-6-phenylphenanthridini um) cation, formed at a ratio of 1 homodimer per 4 or 5 base pairs, is… Expand
Design of microarray probes for virus identification and detection of emerging viruses at the genus level
We developed a computational method that identifies the conserved viral sequences at the genus level for all viral genomes available in GenBank, and established a virus probe library. Expand
A multivariate prediction model for microarray cross-hybridization
- Yian A. Chen, C. Chou, +5 authors J. Almeida
- Computer Science, Medicine
- BMC Bioinformatics
- 1 March 2006
We propose a systematic search strategy using multiple multivariate models [multiple linear regressions, regression trees, and artificial neural network analyses (ANNs)] to select an effective set of predictors for hybridization. Expand
Profiling expression patterns and isolating differentially expressed genes by cDNA microarray system with colorimetry detection.
A high-density cDNA microarray with colorimetry detection system to simultaneously monitor the expression of many genes on nylon membrane is described and characterized. To quantify the expression of… Expand
Tissue Remodeling of Rat Pulmonary Artery in Hypoxic Breathing. I. Changes of Morphology, Zero-Stress State, and Gene Expression
- W. Huang, Y. Sher, David Delgado-West, J. T. Wu, K. Peck, Y. C. Fung
- Medicine, Chemistry
- Annals of Biomedical Engineering
AbstractThe remodeling of the pulmonary arterial tissue in response to a step change of the oxygen concentration in the gas in which a rat lives was recorded as function of time and function of O2… Expand
Design and Assessment of A Fast Algorithm for Identifying Specific Probes for Human and Mouse Genes
We have developed a fast algorithm as well as a software program based on the algorithm for identifying gene specific probes of complex organisms for large databases such as TIGR human THC and mouse TC databases. Expand
Optimization of high-sensitivity fluorescence detection.
We present general expressions for the number of photons emitted by a fluorescent chromophore as a function of the intensity and the duration of illumination. The aim is to find optimal conditions… Expand