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Novel hepatocyte growth factor (HGF) binding domains on fibronectin and vitronectin coordinate a distinct and amplified Met-integrin induced signalling pathway in endothelial cells
These studies identify a novel mechanism and pathway of HGF signalling in endothelial cells involving cooperation between Met and integrins in a Ras dependent manner and have implications for the regulation of neovascularization in both health and disease. Expand
Improved methodology for detecting bromodeoxyuridine in cultured cells and tissue sections by immunocytochemistry
It is found that microwave treatment can allow considerably lower antibody concentrations and eliminates the need for any other denaturation step, which reduces the non-specific background staining found when using monoclonal antibodies on mouse tissue. Expand
Prostaglandin E2 production and metabolism in human breast cancer cells and breast fibroblasts. Regulation by inflammatory mediators.
Breast fibroblasts, particularly under the influence of inflammatory mediators, provide a potentially rich source for PGE2 production in breast tumours, whereas significant contributions from the epithelial tumour component may be restricted to cancer cells exhibiting an invasive phenotype. Expand
Human breast cancer cells contain a phosphoramidon-sensitive metalloproteinase which can process exogenous big endothelin-1 to endothelin-1: a proposed mitogen for human breast fibroblasts.
The results indicate that human breast cancer cells contain a neutral phosphoramidon-sensitive metalloproteinase which can process big ET-1 to ET- 1, and could contribute substantially to the local extracellular levels of this proposed paracrine breast fibroblast mitogen. Expand
Bombesin and glucocorticoids stimulate human breast cancer cells to produce endothelin, a paracrine mitogen for breast stromal cells.
In vitro study demonstrates the potential for bombesin and glucocorticoid to regulate ET production in human breast cancer cells, which may in turn have a paracrine influence on neighboring stromal cell function. Expand
Activation of inositol phospholipid signaling and Ca2+ efflux in human breast cancer cells by bombesin.
Data implicate a functional role for BRPs in human breast cancer by studying their effect on phospholipid hydrolysis, 45Ca2+ efflux, and cell growth in thehuman breast cancer cell line MCF-7 and activation of putative mitogenic signaling pathways. Expand
Evidence for a role for protein kinase C in the modulation of bombesin-activated cellular signalling in human breast cancer cells
Data support an inhibitory role for PKC in the regulation of phosphoinositide hydrolysis and [Ca2+]i in breast cancer cells and provide a potential mechanism for feedback regulation of this signalling pathway in these cells. Expand
Modulation of inositol lipid hydrolysis in human breast cancer cells by two classes of bombesin antagonist.
  • K. Patel, M. Schrey
  • Biology, Medicine
  • Journal of molecular endocrinology
  • 1 February 1991
Inositol lipid hydrolysis was monitored in the human breast cancer cell line MCF-7 in response to various bombesin (BN) and substance P (SP) analogues to show consistent with the presence of separate but interacting receptors or binding sites for BN and SP analogues, which are coupled to a common signal transduction pathway inhuman breast cancer cells. Expand
Inhibition of DNA synthesis and growth in human breast stromal cells by bradykinin: evidence for independent roles of B1 and B2 receptors in the respective control of cell growth and phospholipid
A potential pathophysiological role for BK as a negative regulator of breast stromal cell growth is highlighted and a B1 receptor-mediated pathway is also implicated. Expand
p53 expression in cultured cells following radioisotope labelling.
C Cultured cells were found to express p53 protein following pulse labelling with radioisotopes, even at low doses normally used for growth and metabolic labelling studies, suggesting some stem cells are exquisitely sensitive to radiation and thus p53 may have evolved as a major regulator of stem cell function. Expand