• Publications
  • Influence
Hydrolysis of γ:ε Isopeptides by Cytosolic Transglutaminases and by Coagulation Factor XIIIa*
It is suggested that transglutaminases may play a dynamic role in biology not only by promoting the formation but also the breaking of Nε-(γ-glutamyl)lysine isopeptides. Expand
Specificity of guinea pig liver transglutaminase for amine substrates.
The results point to the importance of a hydrophobic binding region in the enzyme from where the alkyl side chain reaches into a narrow crevice toward the active center and positions the primary amine of the substrate for attacking the carbonyl group of the acyl enzyme intermediate. Expand
Isolation of transglutaminase-reactive sequences from complex biological systems: a prominent lysine donor sequence in bovine lens.
The dansyl hapten offered special advantages as a "handle" for the rapid isolation of transglutaminase targets even in the complex system of lens cortical homogenate, and unambiguously identifies the known carboxyl-terminal domain, EK-PAVTAAPKK, as the prominent lysine-donating fragment in bovine lens. Expand
New colored and fluorescent amine substrates for activated fibrin stabilizing factor (Factor XIIIa) and for transglutaminase.
Two fluorescent (FITC and 6-chloro-2-methoxyacridine) and an intensely colored derivative of cadaverine were synthesized, in order to enlarge the scope of possibilities for labeling some gamma-glutamine sites in proteins. Expand
A double-headed Gly-Pro-Arg-Pro ligand mimics the functions of the E domain of fibrin for promoting the end-to-end crosslinking of gamma chains by factor XIIIa.
A simple double-headed ligand, bis(Gly-Pro-Arg- Pro-amido)polyethylene glycol, is succeeded in fully replacing the regulatory functions of the large E domains of the native protein, fibrinogen. Expand
5-methyl-5,6,7,8-tetrahydropteroyl oligo-gamma-L-glutamates: synthesis and kinetic studies with methionine synthetase from bovine brain.
The synthesis of 5-methyl-5,6,7,8-tetrahydropteroly tri-, pena-, and heptaglutamate has been accomplished, and a role for the ubiquitous methyltetrahoyl oligoglutamates in mammalian methionine biosynthesis is established. Expand
Conformations of cyclic octapeptides. 1
Synthese et caracterisation de 4 octapeptides cycliques diastereoisomeres, cyclo (L- ou D-Ala-Gly-L-Pro-L ou D-Phe) 2 ; donnees RMN et conformations