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Microscopic detection of thermogenesis in a single HeLa cell.
We report here the technique for detection and measurement of the temperature changes in single cells using a recently devised microthermometer (a glass micropipette filled with the thermosensitive… Expand
Purification of cytoplasmic actin by affinity chromatography using the C-terminal half of gelsolin.
- T. Ohki, C. Ohno, K. Oyama, S. V. Mikhailenko, S. Ishiwata
- Medicine, Biology
- Biochemical and biophysical research…
- 22 May 2009
A new rapid method of the cytoplasmic actin purification, not requiring the use of denaturants or high concentrations of salt, was developed, based on the affinity chromatography using the C-terminal… Expand
Hereditary deficiency of lactate dehydrogenase M-subunit.
A family with a complete deficiency of lactate dehydrogenase M-subunit was investigated. The propositus was an 18-year-old male who complained of exertional pigmenturia and easy fatigue. Marked… Expand
Effects of corticosteroids in preterm sheep on adaptation and sympathoadrenal mechanisms at birth.
- H. Stein, K. Oyama, +4 authors J. Padbury
- Biology, Medicine
- The American journal of physiology
- 1 May 1993
This study investigates the effects of prenatal corticosteroid administration on newborn sympathoadrenal mechanisms involved in postnatal adaptation. Randomly assigned preterm (122-125 days) fetal… Expand
A nanoparticle-based ratiometric and self-calibrated fluorescent thermometer for single living cells.
The homeostasis of body temperature and energy balance is one of the major principles in biology. Nanoscale thermometry of aqueous solutions is a challenging but crucial technique to understand the… Expand
Effect of sample site on fatty acid composition of adipose tissue.
Highly thermosensitive Ca2+ dynamics in a HeLa cell through IP3 receptors
Intracellular Ca2+ distribution and its dynamics are essential for various cellular functions. We show with single HeLa cells that a microscopic heat pulse induces Ca2+ uptake into intracellular… Expand
Prolonged β-Agonist Infusion Does Not Induce Desensitization or Down-Regulation of β-Adrenergic Receptors in Newborn Sheep
ABSTRACT: In adult animals, prolonged β-agonist expo-sore leads to down-regulation of β-adrenergic receptors and deseasitization. Prior evidence from our lab suggests that this may not occur in… Expand
The effects of corticosteroids and thyrotropin-releasing hormone on newborn adaptation and sympathoadrenal mechanisms in preterm sheep.
- H. Stein, A. Martínez, L. Blount, K. Oyama, J. Padbury
- American journal of obstetrics and gynecology
- 1 July 1994
OBJECTIVES We examined the effect of prenatal corticosteroids and thyrotropin-releasing hormone on postnatal adaptation and sympathoadrenal function in preterm lambs. STUDY DESIGN Catheterized… Expand
Cardiac thin filament regulation and the Frank–Starling mechanism
- Fuyu Kobirumaki-Shimozawa, Takahiro Inoue, +5 authors Norio Fukuda
- Medicine, Biology
- The Journal of Physiological Sciences
- 1 May 2014
Abstract The heart has an intrinsic ability to increase systolic force in response to a rise in ventricular filling (the Frank–Starling law of the heart). It is widely accepted that the length… Expand