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Switching gene swi6, involved in repression of silent mating-type loci in fission yeast, encodes a homologue of chromatin-associated proteins from Drosophila and mammals.
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Homologous recombination in the fission yeast Schizosaccharomyces pombe: different requirements for the rhp51+, rhp54+ and rad22+ genes
TLDR
The function of Rhp51 and Rhp54 in damage repair and recombination resembles the role of Rad51 and Rad54 in S. cerevisiae, although the relative contribution of gene conversion events is much higher in a rhp51Δ and a rh p54Δ background.
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The fission yeast rad22 gene, having a function in mating-type switching and repair of DNA damages, encodes a protein homolog to Rad52 of Saccharomyces cerevisiae.
TLDR
Southern blot analyses have shown that the rad22-67 mutant frequently gives rise to deletions in the mating-type region, indicating that the Rad22 protein has a function in the repair of DNA double-strand breaks.
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Constitutive expression of hydrophobin HFB1 from Trichoderma reesei in Pichia pastoris and its pre‐purification by foam separation during cultivation
TLDR
The presented data show that Pichia pastoris is a suitable system for production of constitutively expressed and secreted active hydrophobin, allowing for in situ pre‐purification using foam separation.
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Layer thickness of hydrophobin films leads to oscillation in wettability.
TLDR
It is found that the wettability of protein-coated surfaces strictly depends on the amount of adsorbed protein, as reflected in an oscillation of the contact angles of hydrophobin- coated silicon wafers.
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Cloning of human and mouse genes homologous to RAD52, a yeast gene involved in DNA repair and recombination.
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Identification of a chicken RAD52 homologue suggests conservation of the RAD52 recombination pathway throughout the evolution of higher eukaryotes.
TLDR
High abundance of the mRNA in testis makes it likely that the chicken Rad52 protein plays a role in meiotic recombination and sequence comparison of the chicken and yeast proteins reveals a strongly conserved region between positions 40 and 178 of theChicken Rad52 sequence indicating that this part of the protein is under strong evolutionary pressure.
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An exciton-polariton laser based on biologically produced fluorescent protein
TLDR
The unique molecular structure of eGFP prevents exciton annihilation even at high excitation densities, thus facilitating polariton condensation under conventional nanosecond pumping, and is clearly evidenced by a distinct threshold, an interaction-induced blueshift of the condensate, long-range coherence, and the presence of a second threshold at higher excitation density that is associated with the onset of photon lasing.
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Evidence for the association of yeast mitochondrial ribosomes with Cox11p, a protein required for the CuB site formation of cytochrome c oxidase
TLDR
This work experimentally proves that Cox11p possesses a Nin–Cout topology, with the C-terminal copper-binding domain exposed in the mt intermembrane space, and proposes a model in which the CuB site is co-translationally formed by a transient interaction between Cox 11p and the nascent Cox1p in the intermemBRane space.
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Analysis of DNA repair pathways of Schizosaccharomyces pombe by means of swi-rad double mutants.
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