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Compartmentalized expression of two structurally and functionally distinct 4-coumarate:CoA ligase genes in aspen (Populus tremuloides).
Two functionally and structurally distinct 4CL genes are discovered that are differentially expressed in aspen (Populus tremuloides) that reveal the association of Pt4CL1 with the biosynthesis of guaiacyl-syringyl lignin and the involvement of Pt 4CL2 with other phenylpropanoid formation.
Coniferyl aldehyde 5-hydroxylation and methylation direct syringyl lignin biosynthesis in angiosperms.
Cloned cDNAs encoding a cytochrome P450 monooxygenase and a caffeate O-methyltransferase from sweetgum and COMT converted coniferyl aldehyde to sinapyl alde Hyde, suggesting a CAld5H/COMT-mediated pathway from guaiacyl to syringyl monolignol biosynthesis via conifldehyde that contrasts with the generally accepted route to Sinapate via ferulate.
Site-directed mutagenesis in Arabidopsis using custom-designed zinc finger nucleases
These data demonstrate that an approach using ZFNs can be used for the efficient production of mutant plants for precision reverse genetics, and lack of AtKu80, which plays a role in end-protection of dsDNA breaks, increased error-prone rejoining frequency by 2.6-fold.
Response of plants to water stress
The systems that regulate plant adaptation to water stress through a sophisticated regulatory network are the subject of the current review and molecular mechanisms that plants use to increase stress tolerance, maintain appropriate hormone homeostasis and responses and prevent excess light damage are discussed.
Increased frequency of homologous recombination and T‐DNA integration in Arabidopsis CAF‐1 mutants
Fas mutants exhibited increased levels of DNA double‐strand breaks, a G2‐phase retardation that accelerates endoreduplication, and elevated levels of mRNAs coding for proteins involved in HR—all factors that could also contribute to upregulation of HR frequency in fas mutants.
Receptor-like protein kinase 2 (RPK 2) is a novel factor controlling anther development in Arabidopsis thaliana.
The results suggest that RPK2 controls tapetal cell fate by triggering subsequent tapetum degradation, and that mutating RPk2 impairs normal pollen maturation and anther dehiscence due to disruption of key metabolic pathways.
Isolation and characterization of the RAD54 gene from Arabidopsis thaliana.
  • K. Osakabe, K. Abe, +5 authors S. Toki
  • Biology, Medicine
    The Plant journal : for cell and molecular…
  • 1 December 2006
Cloned the full-length AtRAD54 cDNA sequence showed that the AtRad54 protein was indeed expressed as a protein of approximately 110 kDa in Arabidopsis and pointed to an important role for Rad54 in HR repair in higher plants.
Arabidopsis RAD51C Gene Is Important for Homologous Recombination in Meiosis and Mitosis1
The role of RAD51C in meiotic and mitotic recombination in higher plants is determined by characterized a T-DNA insertion mutant of AtRAD51C, which grew normally during vegetative developmental stage, and produced aborted siliques, and their anthers did not contain mature pollen grains.
Efficient Genome Editing in Apple Using a CRISPR/Cas9 system
This study describes induction of a targeted gene mutation in the endogenous apple phytoene desaturase (PDS) gene using the CRISPR/Cas9 system, and demonstrates that genome editing can be practically applied to modify the apple genome.
Overproduction of the Membrane-bound Receptor-like Protein Kinase 1, RPK1, Enhances Abiotic Stress Tolerance in Arabidopsis*
The results suggest that the overproduction of RPK1 enhances both the ABA and drought stress signaling pathways and enhances both water and oxidative stress tolerance in Arabidopsis.