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Stacks on tracks: the plant Golgi apparatus traffics on an actin/ER network.
We have visualized the relationship between the endoplasmic reticulum (ER) and Golgi in leaf cells of Nicotiana clevelandii by expression of two Golgi proteins fused to green fluorescent proteinExpand
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Functional Analysis of a DNA-Shuffled Movement Protein Reveals That Microtubules Are Dispensable for the Cell-to-Cell Movement of Tobacco mosaic virus Article, publication date, and citation
Microtubules interact strongly with the viral movement protein (MP) of Tobacco mosaic virus (TMV) and are thought to transport the viral genome between plant cells. We describe a functionallyExpand
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Two Plant–Viral Movement Proteins Traffic in the Endocytic Recycling Pathwayw⃞
Many plant viruses exploit a conserved group of proteins known as the triple gene block (TGB) for cell-to-cell movement. Here, we investigated the interaction of two TGB proteins (TGB2 and TGB3) ofExpand
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Simple, but Not Branched, Plasmodesmata Allow the Nonspecific Trafficking of Proteins in Developing Tobacco Leaves
Leaves undergo a sink-source transition during which a physiological change occurs from carbon import to export. In sink leaves, biolistic bombardment of plasmids encoding GFP-fusion proteinsExpand
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Phloem Unloading in Sink Leaves of Nicotiana benthamiana: Comparison of a Fluorescent Solute with a Fluorescent Virus.
Using noninvasive imaging techniques, we compared phloem unloading of the membrane-impermeant, fluorescent solute carboxyfluorescein (CF) with that of potato virus X expressing the gene for the greenExpand
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The photoreversible fluorescent protein iLOV outperforms GFP as a reporter of plant virus infection
Fluorescent proteins (FPs) based on green fluorescent protein (GFP) are widely used throughout cell biology to study protein dynamics, and have extensive use as reporters of virus infection andExpand
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Expression of GFP-fusions in Arabidopsis companion cells reveals non-specific protein trafficking into sieve elements and identifies a novel post-phloem domain in roots.
Transgenic Arabidopsis plants were constructed to express a range of GFP-fusion proteins (36-67 kDa) under the companion cell (CC)-specific AtSUC2 promoter. These plants were used to monitor theExpand
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Plasmodesmata and the control of symplastic transport
In 1879 Eduard Tangle discovered cytoplasmic connections between cells in the cotyledons of Strychnos nuxvomica , which he interpreted to be protoplasmic contacts. This led him to hypothesize thatExpand
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Gating of epidermal plasmodesmata is restricted to the leading edge of expanding infection sites of tobacco mosaic virus (TMV).
Plasmodesmatal gating in epidermal cells of Nicotiana tabacum was examined in expanding infection sites of tobacco mosaic virus (TMV) expressing a fusion between the viral movement protein and theExpand
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Getting the message across: how do plant cells exchange macromolecular complexes?
  • K. Oparka
  • Medicine, Biology
  • Trends in plant science
  • 2004
A major pathway for macromolecular exchange in plants involves plasmodesmata (PD), the small pores that connect adjoining cells. This article considers the nature of macromolecular complexes (MCs)Expand
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