Generation of germline-competent induced pluripotent stem cells
iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application.
Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
A modified protocol for the generation of iPS cells that does not require the Myc retrovirus is described and, with this protocol, significantly fewer non-iPS background cells are obtained, and theiPS cells generated were consistently of high quality.
A more efficient method to generate integration-free human iPS cells
A simple method is reported, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors, which may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future.
Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors
The production of virus-free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of i PS cells in regenerative medicine.
Induction of pluripotent stem cells from fibroblast cultures
Detailed methods and tips for the generation of induced pluripotent stem cells, named iPS cells, can be differentiated into three germ layers and committed to chimeric mice are described.
Suppression of induced pluripotent stem cell generation by the p53–p21 pathway
Functional analyses of 34 p53-regulated genes demonstrate that the p53–p21 pathway serves as a barrier not only in tumorigenicity, but also in iPS cell generation in mouse and human fibroblasts.
Immunogenicity of induced pluripotent stem cells.
A new study shows the immunogenicity of induced pluripotent stem cells by the direct transplantation of undifferentiated cells into syngenic mice, indicating that cells differentiated from iPSCs can at least partially replace the biological functions of various organs.
Generation of Pluripotent Stem Cells from Adult Mouse Liver and Stomach Cells
Genetic lineage tracings suggest that iPS cells are generated by direct reprogramming of lineage-committed somatic cells and that retroviral integration into specific sites is not required.
Drug Screening for ALS Using Patient-Specific Induced Pluripotent Stem Cells
The new work provides an encouraging step toward using motor neurons generated from iPSCs derived from ALS patients to learn more about what triggers the death of motor neurons in this disease and to identify new candidate drugs that may be able to slow or reverse the devastating loss ofMotor neurons.