Share This Author
Molecular phylogeny of parabasalids inferred from small subunit rRNA sequences, with emphasis on the Hypermastigea.
Phylogenetic Identification of Hypermastigotes, Pseudotrichonympha, Spirotrichonympha, Holomastigotoides, and Parabasalian Symbionts in the Hindgut of Termites
The phylogenetic diversity of parabasalian flagellates was examined based on the sequences of small subunit ribosomal RNA genes amplified directly from the mixed population of flageLLates in the hindgut of lower termites, suggesting that the Pseudotrichonympha group is the most probable ancient lineage ofParabasalids and the Trichonympha Group is the secondly deep-branching lineage.
Diverse genes of cellulase homologues of glycosyl hydrolase family 45 from the symbiotic protists in the hindgut of the termite Reticulitermes speratus
- K. Ohtoko, M. Ohkuma, S. Moriya, Tetsushi Inoue, R. Usami, T. Kudo
- 1 December 2000
The presence of diverse cellulase homologues suggests that symbiotic protists of termites may be rich reservoirs of novel cellulase sequences.
Phylogenetic Identification of the Symbiotic Hypermastigote Trichonympha agilis in the Hindgut of the Termite Reticulitermes speratus Based on Small‐Subunit rRNA Sequence
- M. Ohkuma, K. Ohtoko, C. Grunau, S. Moriya, T. Kudo
- BiologyJournal of Eukaryotic Microbiology
- 1 July 1998
The phylogeny of a symbiotic hypermastigote Trichonympha agilis (class Parabasalia; order Hypermastigida) in the hindgut of the lower termite Reticulitermes speratus was examined by a strategy that does not rely on cultivation, indicating that this hyper mastigote and trichomonads shared a recent common ancestry.
Vector-capping: a simple method for preparing a high-quality full-length cDNA library.
A simple method for constructing a full-length cDNA library, which consists of only three steps including direct ligation between a vector and a cDNA strand using T4 RNA ligase, which will accelerate comprehensive gene analysis in a variety of eukaryotes.
Determination of the capped site sequence of mRNA based on the detection of cap-dependent nucleotide addition using an anchor ligation method.
- H. Ohtake, K. Ohtoko, Y. Ishimaru, S. Kato
- Biology, ChemistryDNA research : an international journal for rapid…
Whether or not the cDNA starts from the capped site sequence of mRNA based on the presence or absence of an additional dGMP at the 5' end of the c DNA synthesized using the anchor ligation method is suggested.
Molecular phylogenetic identification of the intestinal anaerobic microbial community in the hindgut of the termite, Reticulitermes speratus, without cultivation
The phylogenetic analysis of the nifH amino acid sequences showed that there was a remarkable diversity of nitrogenase genes in the termite gut, and according to the phylogenetic trees constructed, the hypermastigote represented one of the deepest branches of eukaryotes.
Characterization of malate dehydrogenase from deep-sea psychrophilic Vibrio sp. strain no. 5710 and cloning of its gene.
A metabolic key enzyme malate dehydrogenase (MDH) was purified from a deep-sea psychrophilic bacterium and revealed its primary sequence was similar to that of a mesophile E. coli MDH (78% amino acid identity), for which the three-dimensional structure is known.
Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method.
Full-length transcriptome analysis of these cDNA libraries revealed that there were, unexpectedly, many transcript variants for each gene, indicating that obtaining the full-length cDNA for each variant is indispensable for analyzing its function.
Mutation screening and haplotype analysis of the rhodopsin gene locus in Japanese patients with retinitis pigmentosa
No mutation causing an amino acid substitution of RHO was observed in 68 Japanese patients with RP, but 1 control subject did have a novel amino acids substitution and the RP-associated haplotype was not identified.