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Alcohol oxidases of Kloeckera sp. and Hansenula polymorpha. Catalytic properties and subunit structures.
TLDR
Alcohol oxidase (alcohol: oxygen oxidoreductase) of a thermophilic methanol-utilizing yeast, Hansenula polymorpha DL-1, was isolated in crystalline form and was more stable to heat than was the enzyme of Kloeckera sp.polymorpha, compatible with the difference in growth temperatures for both yeasts. Expand
Tyrosine phenol lyase. I. Purification, crystallization, and properties.
TLDR
Crystalline l-tyrosine phenol lyase (deaminating) was prepared from the cell extract of Escherichia intermedia A-21 grown in a medium supplemented with l-TYrosine and catalyzed the stoichiometric conversion of l-Tyrosine into phenol, pyruvate, and ammonia, in the presence of added pyridoxal phosphate. Expand
Arginine racemase of Pseudomonas graveolens. I. Purification, crystallization, and properties.
TLDR
Arginine racemase has been purified approximately 5400-fold and crystallized from an extract of Pseudomonas graveolens and is homogeneous by the criteria of ultracentrifugation and disc gel electrophoresis. Expand
The Microbial Metabolism of Methanol
A methanol-oxidizing enzyme was extracted from a methanol-utilizing yeast, Kloeckera sp. No. 2201, and was found to be independent of the addition of a hydrogen acceptor. The enzyme, which wasExpand
Characterization of choline dehydrogenase from Pseudomonas aeruginosa A-16.
A choline dehydrogenase, which was present in the particulate fraction of the cell-free extract of Pseudomonas aeruginosa A-16, oxidized choline to betaine aldehyde without any dissociable coenzymes,Expand
The Microbial Metabolism of Methanol Part II
A methanol-oxidizing enzyme was extracted from a methanol-utilizing yeast, Kloeckera sp. No. 2201, and was found to be independent of the addition of a hydrogen acceptor. The enzyme, which wasExpand
Production and Purification of Choline Oxidase from Cylindrocarpon didymum M–1
The distribution of choline oxidase activity was studied with cell-free extracts of yeasts, molds and actinomycetes. A fungus which was identified as Cylindrocarpon didymum M–1 showed the highestExpand
Purification and Characterization of Formaldehyde Dehydrogenase in a Methanol-utilizing Yeast, Kloeckera sp. No. 2201
An NAD-linked formaldehyde dehydrogenating enzyme was found in the cell-extract of Kloeckera sp. No. 2201, which utilized methanol as a sole source of carbon. The enzyme was inducibly formed inExpand
Bacterial oxidation of polyethylene glycol.
TLDR
The metabolism of polyethylene glycol was investigated with a synergistic, mixed culture of Flavobacterium and Pseudomonas species, which are individually unable to utilize PEGs, and the following metabolic pathway was proposed for PEG. Expand
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