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Neuroglobin, nitric oxide, and oxygen: functional pathways and conformational changes.
TLDR
Based on the ligand-linked conformational changes discovered by crystallography, the pathways of the reactions with O2 and NO provide a framework that may account for the involvement of Ngb in controlling the activation of a protective signaling mechanism. Expand
Engineered nanoparticles interacting with cells: size matters
TLDR
Common techniques to characterize NP size are summarized, recent work on the impact of NP size on active and passive cellular internalization and intracellular localization are highlighted and Cytotoxic effects are discussed. Expand
Structural basis for photo-induced protein cleavage and green-to-red conversion of fluorescent protein EosFP.
TLDR
X-ray structures of the green and red forms of WT EosFP reveal that formation of the red chromophore is associated with cleavage of the peptide backbone, with surprisingly little change elsewhere in the structure, and provide insights into the mechanism that generates this interesting posttranslational polypeptide modification. Expand
Structural basis of enhanced photoconversion yield in green fluorescent protein-like protein Dendra2.
TLDR
The structure, which is very similar to those reported for the closely related proteins EosFP and Kaede, revealed a local structural change involving mainly Arg66 and a water molecule W4, which are part of a charged and hydrogen-bonded cluster of amino acids and water molecules next to the chromophore. Expand
Structural characterization of IrisFP, an optical highlighter undergoing multiple photo-induced transformations
TLDR
Crystallography and spectroscopy are combined to characterize the Phe-173-Ser mutant of the tetrameric variant of EosFP, named IrisFP, which incorporates both types of phototransformations and displays altogether 3 distinct photoactivation processes. Expand
Fluorescent proteins for live-cell imaging with super-resolution.
TLDR
There is still room for further improvement of these important markers for live cell imaging, and special FP variants with low switching fatigue have been introduced in recent years. Expand
mRuby, a Bright Monomeric Red Fluorescent Protein for Labeling of Subcellular Structures
TLDR
It is discovered that ordered inheritance of peroxisomes is widespread during mitosis of different mammalian cell types and ordered partitioning is realized by the formation ofPeroxisome clusters around the poles of the mitotic spindle and ensures that equal numbers of the organelle are inherited by the daughter cells. Expand
Optimized and far-red-emitting variants of fluorescent protein eqFP611.
TLDR
The bright fluorescence makes the red-shifted eqFP611 variants promising lead structures for the development of near-infrared fluorescent markers, including two-photon imaging. Expand
Ligand binding and protein dynamics in neuroglobin
TLDR
The ligand-binding reaction over a wide temperature range (3–353 K) is investigated and it is suggested that Ngb fluctuates among different conformations on surprisingly long time scales. Expand
Photoconvertible Fluorescent Protein EosFP: Biophysical Properties and Cell Biology Applications
TLDR
A variety of applications in cellular imaging, developmental biology and automated high-content screening applications are presented, which demonstrate that EosFP is a powerful tool for in vivo monitoring of cellular processes. Expand
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