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Neuroglobin, nitric oxide, and oxygen: functional pathways and conformational changes.
- M. Brunori, A. Giuffrè, K. Nienhaus, G. Nienhaus, F. Scandurra, B. Vallone
- ChemistryProceedings of the National Academy of Sciences…
- 14 June 2005
Based on the ligand-linked conformational changes discovered by crystallography, the pathways of the reactions with O2 and NO provide a framework that may account for the involvement of Ngb in controlling the activation of a protective signaling mechanism.
Engineered nanoparticles interacting with cells: size matters
Common techniques to characterize NP size are summarized, recent work on the impact of NP size on active and passive cellular internalization and intracellular localization are highlighted and Cytotoxic effects are discussed.
Structural basis for photo-induced protein cleavage and green-to-red conversion of fluorescent protein EosFP.
- K. Nienhaus, G. Nienhaus, J. Wiedenmann, H. Nar
- BiologyProceedings of the National Academy of Sciences…
- 28 June 2005
X-ray structures of the green and red forms of WT EosFP reveal that formation of the red chromophore is associated with cleavage of the peptide backbone, with surprisingly little change elsewhere in the structure, and provide insights into the mechanism that generates this interesting posttranslational polypeptide modification.
Structural basis of enhanced photoconversion yield in green fluorescent protein-like protein Dendra2.
The structure, which is very similar to those reported for the closely related proteins EosFP and Kaede, revealed a local structural change involving mainly Arg66 and a water molecule W4, which are part of a charged and hydrogen-bonded cluster of amino acids and water molecules next to the chromophore.
Structural characterization of IrisFP, an optical highlighter undergoing multiple photo-induced transformations
- V. Adam, M. Lelimousin, D. Bourgeois
- Chemistry, BiologyProceedings of the National Academy of Sciences
- 25 November 2008
Crystallography and spectroscopy are combined to characterize the Phe-173-Ser mutant of the tetrameric variant of EosFP, named IrisFP, which incorporates both types of phototransformations and displays altogether 3 distinct photoactivation processes.
mRuby, a Bright Monomeric Red Fluorescent Protein for Labeling of Subcellular Structures
It is discovered that ordered inheritance of peroxisomes is widespread during mitosis of different mammalian cell types and ordered partitioning is realized by the formation ofPeroxisome clusters around the poles of the mitotic spindle and ensures that equal numbers of the organelle are inherited by the daughter cells.
Ligand binding and protein dynamics in neuroglobin
- Jan M. Kriegl, A. Bhattacharyya, K. Nienhaus, Pengchi Deng, Oleksandr Minkow, G. Nienhaus
- Chemistry, BiologyProceedings of the National Academy of Sciences…
- 4 June 2002
The ligand-binding reaction over a wide temperature range (3–353 K) is investigated and it is suggested that Ngb fluctuates among different conformations on surprisingly long time scales.
Optimized and far-red-emitting variants of fluorescent protein eqFP611.
Fluorescent proteins for live-cell imaging with super-resolution.
There is still room for further improvement of these important markers for live cell imaging, and special FP variants with low switching fatigue have been introduced in recent years.
The structure of murine neuroglobin: Novel pathways for ligand migration and binding
The crystal structure of murine ferric (met) neuroglobin at 1.5 Å reveals interesting features relevant to the ligand binding mechanism, and further structure‐function studies can be planned to elucidate the role of Neuroglobin in physiological responses to hypoxia.