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Sorbitol dehydrogenase from Bacillus subtilis. Purification, characterization, and gene cloning.
Cloning of the sorbitol dehydrogenase gene (gutB) from Bacillus subtilis offers an excellent system for studying zinc binding, substrate specificity, and catalytic mechanism of this enzyme throughExpand
Rat chondrosarcoma ATP sulfurylase and adenosine 5'-phosphosulfate kinase reside on a single bifunctional protein.
The results all suggest that ATP sulfurylase and APS kinase from rat chondrosarcoma residue on a single bifunctional protein. Expand
Interaction between pyridoxal kinase and pyridoxal-5-phosphate-dependent enzymes.
The results demonstrated that PK can specifically bind to ALT and GAD, and support the hypothesis that PLP transfer from PK to PLP-dependent enzymes requires a specific interaction between PK and the enzyme. Expand
Sulfate activation and transport in mammals: system components and mechanisms.
Isolation of the PAPS-synthesizing activities from rat chondrosarcoma and preparation of stable non-hydrolyzable analogs of APS and PAPS have facilitated the kinetic characterization of mammalian ATP sulfurylase and APS kinase. Expand
Kinetic mechanism of adenosine 5'-phosphosulphate kinase from rat chondrosarcoma.
Results are in accord with the formulation of the predominant pathway as a steady-state ordered mechanism with APS as the leading substrate and PAPS as the final product released. Expand
Kinetic mechanism of ATP-sulphurylase from rat chondrosarcoma.
The results of steady-state experiments in which Mg2+ was varied indicated that the true substrate is the MgPPi complex, and an analogue of APS, adenosine 5'-[beta-methylene]phosphosulphate, was a linear inhibitor competitive with APS and non-competitive with respect to Mg PPi. Expand
Synthesis and properties of a nonhydrolyzable adenosine phosphosulfate analog.
This paper describes the simple and efficient synthesis and structural confirmation of a nonhydrolyzable APS analog, beta-methylene APS, with an overall molar yield of 40-50%. Expand
Dibasic amines as competitive ions improve the resolution between polyanionic nucleotides.
The ability to easily resolve these positional isomers allows the use of a more simplified synthetic procedure that does not involve the use selective protecting groups to specifically phosphorylate the 2' or 3' hydroxyl group. Expand
Synthesis and utilization of a nonhydrolyzable phosphoadenosine phosphosulfate analog.
The synthesis of the 2'- and 3'-isomers of a nonhydrolysable, and thus stable, PAPS analog, beta-methylene-PAPS, from the previously described beta- methylene-APS is described. Expand