K. Nakano

Publications112
h-index27
Citations2,141
Highly Influential Citations65
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Molecular cloning and characterization of a novel Ste20-related protein kinase enriched in neurons and transporting epithelia.
A novel cDNA encoding a protein kinase (termed PASK) was isolated from rat brain. The PASK catalytic domain was most similar to Ste20-related protein kinases, showing 45.5 and 39.2% amino acid… Expand
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The complete amino acid sequence of potato alpha-glucan phosphorylase.
The complete amino acid sequence of potato alpha-glucan phosphorylase has been determined. The monomer contains 916 amino acids with a molecular weight of 103,916. About one-fourth of the… Expand
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Mouse group X secretory phospholipase A2 induces a potent release of arachidonic acid from spleen cells and acts as a ligand for the phospholipase A2 receptor.
Group X secretory phospholipase A2 (sPLA2-X) has recently been shown to possess a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Here, we report the purification of… Expand
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Amino Acid Residue Penultimate to the Amino-terminal Gly Residue Strongly Affects Two Cotranslational Protein Modifications, N-Myristoylation andN-Acetylation*
To examine the amino-terminal sequence requirements for cotranslational protein N-myristoylation, a series of site-directed mutagenesis of N-terminal region were performed using tumor necrosis factor… Expand
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C‐terminal 15 kDa fragment of cytoskeletal actin is posttranslationally N‐myristoylated upon caspase‐mediated cleavage and targeted to mitochondria
To detect the posttranslational N‐myristoylation of caspase substrates, the susceptibility of the newly exposed N‐terminus of known caspase substrates to protein N‐myristoylation was evaluated by in… Expand
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Human muscle glycogen synthase cDNA sequence: a negatively charged protein with an asymmetric charge distribution.
The cDNA for human muscle glycogen synthase encodes a protein of 737 amino acids. The primary structure of glycogen synthase is not related either to bacterial glycogen synthase or to any glycogen… Expand
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Scanning force microscopy of the interaction events between a single molecule of heavy meromyosin and actin.
How close an approach is necessary for two interactive protein molecules to recognize each other before association? How strong a force field is exerted between two proteins at the recognition… Expand
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Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation.
In order to determine the amino-terminal sequence requirements for protein N-myristoylation, site-directed mutagenesis of the N-terminal region was performed using tumor necrosis factor (TNF) mutants… Expand
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Proline- and Alanine-rich Ste20-related Kinase Associates with F-actin and Translocates from the Cytosol to Cytoskeleton upon Cellular Stresses*
Proline- and alanine-rich Ste20-related kinase (PASK) is a Ste20-related protein kinase isolated from rat brain. Cell fractionation studies showed that PASK was present both in the cytosol and in… Expand
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Purified Group X Secretory Phospholipase A2 Induced Prominent Release of Arachidonic Acid from Human Myeloid Leukemia Cells*
Group X secretory phospholipase A2 (sPLA2-X) possesses several structural features characteristic of both group IB and IIA sPLA2s (sPLA2-IB and -IIA) and is postulated to be involved in inflammatory… Expand
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