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Isolation and one-step preparation of A2E and iso-A2E, fluorophores from human retinal pigment epithelium.
- C. Parish, M. Hashimoto, K. Nakanishi, J. Dillon, J. Sparrow
- BiologyProceedings of the National Academy of Sciences…
- 8 December 1998
An investigation aimed at optimizing the in vitro synthesis of A2E has resulted in the one-step biomimetic preparation of this pigment in 49% yield, readily producing more than 50 mg in one step, which has allowed for the optimization of HPLC conditions so that nanogram quantities of A1E can be detected from extracts of tissue samples.
The lipofuscin fluorophore A2E mediates blue light-induced damage to retinal pigmented epithelial cells.
A2E is implicate as an initiator of blue light-induced apoptosis of RPE cells, a finding that indicates an apoptotic form of cell death.
A2E, a byproduct of the visual cycle
A2E, a lipofuscin fluorophore, in human retinal pigmented epithelial cells in culture.
- J. Sparrow, C. Parish, M. Hashimoto, K. Nakanishi
- BiologyInvestigative ophthalmology & visual science
- 1 November 1999
The membrane damage exhibited by A 2E-accumulating RPE is dependent on the concentration of A2E and reflects the ability of this amphiphilic compound to exert detergent-like effects.
Circular dichroic spectroscopy : exciton coupling in organic stereochemistry
Isolation and structure of a covalent cross-link adduct between mitomycin C and DNA.
- M. Tomasz, R. Lipman, D. Chowdary, J. Pawlak, G. Verdine, K. Nakanishi
- Chemistry, BiologyScience
- 6 March 1987
A DNA cross-link adduct of the antitumor agent mitomycin C (MC) to DNA has been isolated and characterized; the results provide direct proof for bifunctional alkylation of DNA by MC. Exposure of MC…
Terpene Trilactones from Ginkgo biloba Are Antagonists of Cortical Glycine and GABAA Receptors*
- L. Ivić, T. Sands, N. Fishkin, K. Nakanishi, A. Kriegstein, K. Strømgaard
- Biology, ChemistryJournal of Biological Chemistry
- 5 December 2003
Molecular modeling is employed to elucidate the similarities and differences in the structure of the terpene trilactones to account for the pharmacological properties of these compounds and demonstrate a striking similarity between ginkgolides and picrotoxinin, a GABAA and recombinant glycine α-homomeric receptor antagonist.
Involvement of oxidative mechanisms in blue-light-induced damage to A2E-laden RPE.
- J. Sparrow, Jilin Zhou, S. Ben-Shabat, H. Vollmer, Y. Itagaki, K. Nakanishi
- Chemistry, BiologyInvestigative ophthalmology & visual science
- 1 April 2002
The augmentation of cell death in the presence of D(2)O and the protection afforded by quenchers and scavengers of singlet oxygen, indicates that the generation of Singlet oxygen may be involved in the mechanisms leading to the death of A2E-containing RPE cells after blue light illumination.
Biosynthetic Studies of A2E, a Major Fluorophore of Retinal Pigment Epithelial Lipofuscin*
The detection in outer segments of a phosphodiesterase activity that can convert A2-PE to A2E may indicate that some portion of the A1-PE that forms in the outer segment membrane may undergo hydrolytic cleavage before internalization by the retinal pigment epithelial cell.