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Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli
TLDR
The most important function of carotenoid pigments, especially beta-carotene in higher plants, is to protect organisms against photooxidative damage, and this work has elucidated for the first time the pathway for biosynthesis of theseCarotenoids at the level of enzyme-catalyzed reactions, using bacterial carOTenoid biosynthesis genes.
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Microlunatus phosphovorus gen. nov., sp. nov., a new gram-positive polyphosphate-accumulating bacterium isolated from activated sludge.
TLDR
A phylogenetic analysis based on 16S rRNA sequences showed that the strain NM-1T (T = type strain) should be assigned to a new genus and species, for which the name Microlunatus phosphovorus is proposed.
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A full-scale operation of a novel activated sludge process without excess sludge production
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Syntrophothermus lipocalidus gen. nov., sp. nov., a novel thermophilic, syntrophic, fatty-acid-oxidizing anaerobe which utilizes isobutyrate.
A new anaerobic, thermophilic, syntrophic, fatty-acid-oxidizing bacterium designated strain TGB-C1T was isolated from granular sludge in a thermophilic upflow anaerobic sludge blanket (UASB) reactor.…
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Different sensitivity to wortmannin of two vacuolar sorting signals indicates the presence of distinct sorting machineries in tobacco cells
TLDR
There are at least two different mechanisms for vacuolar sorting in tobacco cells, and the CTPP-mediated pathway is sensitive to wortmannin, a specific inhibitor of mammalian phosphatidylinositol (PI) 3-kinase on vacUolar delivery by NTPP and CTPP in tobacco Cells.
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High catalytic activity of human cytochrome P450 co-expressed with human NADPH-cytochrome P450 reductase in Escherichia coli.
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A Vacuolar-Type H+-ATPase in a Nonvacuolar Organelle Is Required for the Sorting of Soluble Vacuolar Protein Precursors in Tobacco Cells.
TLDR
Observations indicate that the V-ATPase in the organelle that was recovered in the Golgi-enriched fraction is required for the transport of vacuolar protein precursors and that this V-atPase is distinguishable from the tonoplast-associated V- ATPase.
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Phylogenetic diversity of mesophilic and thermophilic granular sludges determined by 16S rRNA gene analysis.
TLDR
The microbial diversity of two types of methanogenic granular sludge, mesophilic and thermophilic, which had been treating sucrose/propionate/acetate-based artificial wastewater were compared, suggesting that the microbial Diversity of the thermophobic granule was lower than that of the Mesophilic granule.
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Novel heme-containing lyase, phenylacetaldoxime dehydratase from Bacillus sp. strain OxB-1: purification, characterization, and molecular cloning of the gene.
TLDR
Genetic evidence for a novel gene cluster that is responsible for aldoxime metabolism in this microorganism is provided, and the enzyme has been tentatively named phenylacetaldoxime dehydratase.
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Propeptide of a precursor to a plant vacuolar protein required for vacuolar targeting.
TLDR
Results indicate that the propeptide of the precursor to sporamin is required for correct targeting of sporamin to the vacuole and that proteins can be secreted from plant cells by a bulk-flow default pathway in the absence of a functional sorting signal.
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