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Crystal structure at 1.92 Å resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin
TLDR
The crystal structure of the RNA-binding domain of the small nuclear ribonucleoprotein U1A bound to a 21-nucleotide RNA hairpin has been determined and reveals the stereochemical basis for sequence-specific RNA recognition by the RNP domain. Expand
Crystal Structures of Two Sm Protein Complexes and Their Implications for the Assembly of the Spliceosomal snRNPs
TLDR
Crystal structures suggest that the seven Sm proteins could form a closed ring and the snRNAs may be bound in the positively charged central hole. Expand
Crystal structure of the RNA-binding domain of the U1 small nuclear ribonucleoprotein A
The crystal structure of the RNA binding domain of the U1 small nuclear ribonucleoprotein A, which forms part of the ribonucleoprotein complex involved in the excision of introns, has been solved. ItExpand
CryoEM structure of the spliceosome immediately after branching
TLDR
The 3.8 Å cryo-electron microscopy structure of the spliceosome immediately after lariat formation is presented, suggesting a plausible mechanism of remodelling before exon ligation. Expand
Crystal structure of the spliceosomal U2B″–U2A′ protein complex bound to a fragment of U2 small nuclear RNA
TLDR
The crystal structure reveals how protein–protein interaction regulates RNA-binding specificity, and how replacing only a few key residues allows the U2B″ and U1A proteins to discriminate between their cognate RNA hairpins by forming alternative networks of interactions. Expand
Induced structural changes of 7SL RNA during the assembly of human signal recognition particle
TLDR
The crystal structure of the ternary complex suggests why SRP19 is necessary for the stable binding of SRP54 to the S domain RNA. Expand
Recognition of a signal peptide by the signal recognition particle
TLDR
A fusion protein is produced between Sulfolobus solfataricus SRP54 and a signal peptide connected via a flexible linker and it is functional, as demonstrated by its ability to bind SRP RNA and SRP receptor FtsY. Expand
Crystal structure of SRP19 in complex with the S domain of SRP RNA and its implication for the assembly of the signal recognition particle.
TLDR
The crystal structure of Methanococcus jannaschii SRP19 bound to the S domain of human 7SL RNA at 2.9 A resolution is determined, which indicates that the particle clamps the tetraloops of two branched helices and allows them to interact side by side. Expand
Solution structure of the N-terminal RNP domain of U1A protein: the role of C-terminal residues in structure stability and RNA binding.
TLDR
The solution structure of a fragment of the human U1A spliceosomal protein determined using multi-dimensional heteronuclear NMR is presented and the C-terminal region of the molecule is considerably more ordered in the free protein than thought previously. Expand
Structure of a pre-catalytic spliceosome
TLDR
The cryo-electron microscopy structure of the yeast Saccharomyces cerevisiae pre-catalytic B complex spliceosome at near-atomic resolution is reported, providing important insights into the events leading to active site formation. Expand
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