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Transformation of intact yeast cells treated with alkali cations.
TLDR
The transformation efficiency with Cs+ or Li+ was comparable with that of conventional protoplast methods for a plasmid containing ars1, although not for plasmids containing a 2 microns origin replication. Expand
Transformation of intact yeast cells treated with alkali cations
TLDR
The transformation efficiency with Cs+ or Li+ was comparable with that of conventional protoplast methods for a plasmid containing ars1, although not for plasmids containing a 2 microns origin replication. Expand
Molecular characterization of Escherichia coli NAD kinase.
TLDR
The deduced amino-acid sequence of YfjB exhibited homology with that of Mycobacterium tuberculosis inorganic polyphosphate/ATP-NAD kinase, and alignment of their sequences revealed highly conserved regions, XXX-XGGDG-XL and DGXXX-TPTGSTAY, where X represents a hydrophobic amino- acid residue. Expand
Crystal Structure of Exotype Alginate Lyase Atu3025 from Agrobacterium tumefaciens*
TLDR
The structural analysis of H531A/ΔGGG and subsequent site-directed mutagenesis studies proposed the enzyme reaction mechanism, with His311 and Tyr365 as the catalytic base and acid, respectively. Expand
A structural basis for depolymerization of alginate by polysaccharide lyase family-7.
TLDR
The crystal structure and results of mutational analysis of Sphingomonas sp. Expand
Structure and Function of NAD Kinase and NADP Phosphatase: Key Enzymes That Regulate the Intracellular Balance of NAD(H) and NADP(H)
  • S. Kawai, K. Murata
  • Biology, Medicine
  • Bioscience, biotechnology, and biochemistry
  • 23 April 2008
TLDR
The physiological functions, applications, and structure of NAD kinase, and the way the way it was discovered archaeal NADP phosphatase are summarized. Expand
Induced-fit motion of a lid loop involved in catalysis in alginate lyase A1-III.
  • B. Mikami, M. Ban, +7 authors K. Murata
  • Chemistry, Medicine
  • Acta crystallographica. Section D, Biological…
  • 1 September 2012
TLDR
The structures of two mutants of a mannuronate-specific alginate lyase, A1-III, from Sphingomonas species A1 complexed with a tetrasaccharide substrate were determined by X-ray crystallography at around 2.2 Å resolution together with the apo form of the H192A mutant. Expand
Molecular Cloning and Identification of N-Acyl-D-glucosamine 2-Epimerase from Porcine Kidney as a Renin-binding Protein*
TLDR
That GlcNAc 2-epimerase is a RnBP was confirmed by its ability to bind porcine kidney renin and mask its protease activity. Expand
Crystal structure of glycoside hydrolase family 78 alpha-L-Rhamnosidase from Bacillus sp. GL1.
TLDR
To the authors' knowledge, this is the first report on the determination of the crystal structure of alpha-L-rhamnosidase and identification of its clan-L (alpha/alpha)(6)-barrel as a catalytic domain. Expand
Plant Cell Wall Degradation by Saprophytic Bacillus subtilis Strains: Gene Clusters Responsible for Rhamnogalacturonan Depolymerization
TLDR
The degradation system of rhamnogalacturonan type I (RG-I), a component of pectin from the plant cell wall, is shown in B. subtilis strain 168, the first report on the bacterial system for complete RG-I main chain degradation in plant-pathogenic fungus Aspergillus aculeatus. Expand
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