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Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified. Expand
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation. Expand
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
Two new methods were used to establish a rapid and highly sensitive prenatal diagnostic test for sickle cell anemia. The first involves the primer-mediated enzymatic amplification of specific… Expand
Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction.
- K. Mullis, F. Faloona, S. Scharf, R. Saiki, G. Horn, H. Erlich
- Chemistry, Medicine
- Cold Spring Harbor symposia on quantitative…
An alternative method for the synthesis of specific DNA sequences is explored that involves the reciprocal interaction of two oligonucleotides and the DNA polymerase extension products whose synthesis they prime, when they are hybridized to different strands of a DNA template in a relative orientation such that their extension products overlap. Expand
Enzymatic amplification of ?-globin genomic sequences and restriction site analysis for diagnosis of
ExoSAP-IT offers quick, one step PCR clean-up with 100% recovery of both short and long PCR products, and is completely scaleable for manual or automated use. Expand
The unusual origin of the polymerase chain reaction.
- K. Mullis
- Physics, Medicine
- Scientific American
- 1 April 1990
I stumbled across a process that could make unlimited numbers of copies of genes, a process now known as the polymerase chain reaction (PCR), one Friday night in April, 1983, as I gripped the steering wheel of my car and snaked along a moonlit mountain road into northern California's redwood country. Expand
Analysis of enzymatically amplified β-globin and HLA-DQα DNA with allele-specific oligonucleotide probes
The polymerase chain reaction (PCR) procedure is used to enzymatically amplify a specific segment of the β-globin or HLA-DQα gene in human genomic DNA before hybridization with ASOs, enabling the analysis of allelic variation with as little as 1 ng of genomic DNA and the use of a simple ‘dot blot’ for probe hybridization. Expand
Analysis of enzymatically amplified beta-globin and HLA-DQ alpha DNA with allele-specific oligonucleotide probes.
The polymerase chain reaction (PCR) procedure is used to enzymatically amplify a specific segment of the beta-globin or HLA-DQ alpha gene in human genomic DNA before hybridization with ASOs, enabling the analysis of allelic variation with as little as 1 ng of genomic DNA and the use of a simple 'dot blot' for probe hybridization. Expand
The Polymerase Chain Reaction
This handbook provides up-to-date methodological protocols from the world's leading laboratories, in addition to new techniques and enhanced applications not yet available in book form. Expand
Target amplification for DNA analysis by the polymerase chain reaction.
- K. Mullis
- Annales de biologie clinique
The polymerase chain reaction (PCR) has become a standard laboratory technique and certain generalities regarding the relative utility of various protocols for carrying out the PCR are described. Expand