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A new method for producing transgenic birds via direct in vivo transfection of primordial germ cells
Traditional methods of avian transgenesis involve complex manipulations involving either retroviral infection of blastoderms or the ex vivo manipulation of primordial germ cells (PGCs) followed byExpand
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Molecular cloning, expression, and characterization of chicken IFN -lambda.
Interferons (IFN) provide a critical first line of defense against viral infection in vertebrates. Moreover, IFN-lambda, a recently identified group of mammalian IFN, has demonstrated antiviralExpand
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Development of an anti-porcine CD34 monoclonal antibody that identifies hematopoietic stem cells.
OBJECTIVES The isolation of porcine hematopoietic stem cells (HSC) would be an important step toward development of porcine-to-human chimerism for induction of tolerance in clinicalExpand
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Transgenic Chickens Overexpressing Aromatase Have High Estrogen Levels but Maintain a Predominantly Male Phenotype.
Estrogens play a key role in sexual differentiation of both the gonads and external traits in birds. The production of estrogen occurs via a well-characterized steroidogenic pathway, which is aExpand
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Generation of gene edited birds in one generation using sperm transfection assisted gene editing (STAGE)
Generating transgenic and gene edited mammals involves in vitro manipulation of oocytes or single cell embryos. Due to the comparative inaccessibility of avian oocytes and single cell embryos, novelExpand
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Overexpression of Anti-Müllerian Hormone Disrupts Gonadal Sex Differentiation, Blocks Sex Hormone Synthesis, and Supports Cell Autonomous Sex Development in the Chicken.
The primary role of Anti-Müllerian hormone (AMH) during mammalian development is the regression of Müllerian ducts in males. This highly conserved function is retained in birds and is supported byExpand
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Expression, purification and characterisation of recombinant Escherichia coli derived chicken interleukin-12.
Zoonotic viruses, such as H5N1 Avian Influenza, pose major threats to both animals and humans, and with this in mind there is a need for the development of new anti-viral strategies. The cytokineExpand
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Expression of biologically active recombinant porcine interleukin-12 from Escherichia coli.
The control of viral infections is of critical importance to livestock industries worldwide and is highlighted by costly infection outbreaks, such as that seen with foot and mouth disease virus. ToExpand
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Gonadal and Endocrine Analysis of a Gynandromorphic Chicken
Birds have a ZZ male and ZW female sex chromosome system. The relative roles of genetics and hormones in regulating avian sexual development have been revealed by studies on gynandromorphs.Expand
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Administration of porcine interleukin-3 induces increased levels of blood eosinophils.
Increasing resistance to anthelmintic drugs indicates a vital need to develop alternative strategies to control helminth infections. Interleukin-3 (IL-3) is a multilineage hematopoietic growthExpand
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