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Cloning, sequencing, overexpression and characterization of l-rhamnose isomerase from Bacillus pallidus Y25 for rare sugar production
- Wayoon Poonperm, G. Takata, H. Okada, K. Morimoto, T. Granström, K. Izumori
- BiologyApplied Microbiology and Biotechnology
- 25 July 2007
The findings indicated that the recombinant l-RhI from B. pallidus is appropriate for use as a new source of rare sugar producing enzyme on a mass scale production.
Cloning, sequencing, and expression of the gene encoding Clostridium paraputrificum chitinase ChiB and analysis of the functions of novel cadherin-like domains and a chitin-binding domain
Deletion analysis showed clearly that the CBD of ChiB plays an important role in hydrolysis of native chitin but not processed chitIn such as colloidal chit in C. paraputrificum.
Overexpression of a hydrogenase gene in Clostridium paraputrificum to enhance hydrogen gas production.
Novel reactions of L-rhamnose isomerase from Pseudomonas stutzeri and its relation with D-xylose isomerase via substrate specificity.
L-Xylose and L-lyxose production from xylitol using Alcaligenes 701B strain and immobilized L-rhamnose isomerase enzyme
Conversion of chitinous wastes to hydrogen gas by Clostridium paraputrificum M-21.
Efficient biosynthesis of D-allose from D-psicose by cross-linked recombinant L-rhamnose isomerase: separation of product by ethanol crystallization.
- Buetusiwa Thomas Menavuvu, Wayoon Poonperm, K. Izumori
- BiologyJournal of bioscience and bioengineering
- 1 April 2006
Mass production of a rare aldohexose D-allose from D-psicose was achieved in a batch reaction by crude recombinant L-rhamnose isomerase (L-RhI) cross-linked with glutaraldehyde, which had an operative half-life of two months after repeated usages.
A novel β-N-acetylglucosaminidase of Clostridiumparaputrificum M-21 with high activity on chitobiose
SDS-PAGE, zymogram, and immunological analyses suggested that this enzyme is induced by ball-milled chitin, and was optimally active at 50°C and pH 7.0.
Identification and characterization of Clostridium paraputrificum M-21, a chitinolytic, mesophilic and hydrogen-producing bacterium.