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Programmable Ligand Detection System in Plants through a Synthetic Signal Transduction Pathway
TLDR
This work describes assembly and function of a complete synthetic signal transduction pathway in plants that links input from computationally re-designed periplasmic binding proteins to a visual response and may allow plants to serve as a simple and inexpensive means to monitor human surroundings for substances such as pollutants, explosives, or chemical agents.
Engineering key components in a synthetic eukaryotic signal transduction pathway
TLDR
It is shown that sequence conservation and cross talk can extend across kingdoms and can be exploited to produce a synthetic plant signal transduction system.
Computational design of environmental sensors for the potent opioid fentanyl
TLDR
The computational design of proteins that bind the potent analgesic fentanyl is described, using the designs to generate plant sensors for fentanyl by coupling ligand binding to design stability and should be generally useful for detecting toxic hydrophobic compounds in the environment.
Cytosolic glutamine synthetase in soybean is encoded by a multigene family, and the members are regulated in an organ-specific and developmental manner.
TLDR
Genomic Southern analysis and analysis of hybrid-select translation products suggest that each member of the three GS1 gene classes may be derived from the two ancestral genomes from which the allotetraploid soybean was derived.
A general strategy to construct small molecule biosensors in eukaryotes
TLDR
The biosensors based on a ligand-binding domain (LBD) are produced by using a method that, in principle, can be applied to any target molecule and used to improve the biotransformation of pregnenolone to progesterone in yeast and to regulate CRISPR activity in mammalian cells.
Plant viral intergenic DNA sequence repeats with transcription enhancing activity
TLDR
A large set of viral intergenic regions for transcriptional enhancers is 'mined' as a first step in a broad screen of geminivirus and nanovirus intergenic sequences for DNA segments important in controlling viral gene expression.
A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity.
TLDR
A synthetic de-greening circuit is designed that produced rapid chlorophyll loss on perception of a specific input, providing the first easily re- settable reporter system for plants and the capacity to make re-settable biosensors.
Engineering synthetic regulatory circuits in plants.
Crosstalk between endogenous and synthetic components – synthetic signaling meets endogenous components
TLDR
In this review, the design and function of two partially synthetic signaling pathways for use in plants is discussed and observed interactions (crosstalk) with endogenous signaling components are described.
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