Share This Author
Phosphorylation and regulation of Raf by Akt (protein kinase B).
Observations provide a molecular basis for cross talk between two signaling pathways at the level of Raf and Akt in a human breast cancer cell line.
Differentiation stage-specific inhibition of the Raf-MEK-ERK pathway by Akt.
The stage-specific inhibitory action of Akt correlated with its stage- specific ability to form a complex with Raf, suggesting the existence of differentially expressed mediators of an inhibitory Akt-Raf complex.
Therapy of tuberculosis in mice by DNA vaccination
It is shown in mice that DNA vaccines, initially designed to prevent infection, can also have a pronounced therapeutic action, and in heavily infected mice, DNA vaccinations can switch the immune response from one that is relatively inefficient and gives bacterial stasis to one that kills bacteria.
Regulation of Raf-Akt Cross-talk
- K. Moelling, K. Schad, M. Bosse, S. Zimmermann, Marc Schweneker
- BiologyThe Journal of Biological Chemistry
- 23 August 2002
We have recently shown that the Ras-Raf-MEK-ERK and phosphatidylinositol 3-kinase (PI3K)-Akt signaling pathways can cross-talk in the human breast cancer cell line MCF-7. High Raf activity induces…
The Junction-associated Protein AF-6 Interacts and Clusters with Specific Eph Receptor Tyrosine Kinases at Specialized Sites of Cell–Cell Contact in the Brain
AF-6 is a candidate for mediating the clustering of Eph receptors at postsynaptic specializations in the adult rat brain.
Specific binding of HIV‐1 nucleocapsid protein to PSI RNA in vitro requires N‐terminal zinc finger and flanking basic amino acid residues.
This work demonstrates for the first time specific interaction of a retroviral NC protein with its PSI RNA in vitro and demonstrates that chemically synthesized NCp7 specifically binds to viral RNA containing the PSI using competitive filter binding assays.
The Bcr Kinase Downregulates Ras Signaling by Phosphorylating AF-6 and Binding to Its PDZ Domain
- G. Radziwill, R. A. Erdmann, U. Margelisch, K. Moelling
- Biology, ChemistryMolecular and Cellular Biology
- 1 July 2003
It is suggested that AF-6 functions as a scaffold-like protein that links Bcr and Ras to cellular junctions and is involved in downregulation of Ras-mediated signaling at sites of cell-cell contact to maintain cells in a nonproliferating state.
The amino terminus of Tax is required for interaction with the cyclic AMP response element binding protein
Tax of human T-cell leukemia virus type 1 was analyzed for interaction with the cyclic AMP response element binding protein (CREB) in vitro with and without Tax response element DNA and the amino terminus of Tax is essential for Tax-CREB interaction.
Identification and characterization of HIV‐specific RNase H by monoclonal antibody.
It is demonstrated that the HIV‐reverse transcriptase, which consists of a two‐polypeptide complex, p66 and p51, copurifies with an RNase H activity which exhibits properties of a processive exonuclease.
Activated Ras displaces 14-3-3 protein from the amino terminus of c-Raf-1.
Bivalent binding of 14-3-3 zeta to the amino terminus as well as to the carboxy terminus of c-Raf-1 is indicated in unstimulated cells.