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A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
The development of an improved version of YFP named Venus, which contains a novel mutation, F46L, which at 37°C greatly accelerates oxidation of the chromophore, the rate-limiting step of maturation and will enable fluorescent labelings that were not possible before.
Calcium stores regulate the polarity and input specificity of synaptic modification
Reduction of postsynaptic calcium influx by partial blockade of NMDA (N-methyl-d-aspartate) receptors results in a conversion of LTP to LTD and a loss of input specificity normally associated with LTP, with LTD appearing at heterosynaptic inputs.
2APB, 2-aminoethoxydiphenyl borate, a membrane-penetrable modulator of Ins(1,4,5)P3-induced Ca2+ release.
- T. Maruyama, T. Kanaji, S. Nakade, T. Kanno, K. Mikoshiba
- Biology, ChemistryJournal of biochemistry
- 1 September 1997
Although the specificity of 2APB with respect to the intracellular signaling system was not fully established, it is the first candidate for a membrane-penetrable modulator of Ins(1,4,5)P3 receptor, and it should be a useful tool to investigate the physiological role of the Ins(2,3,3)P2 receptor in various cells.
The Neural RNA-Binding Protein Musashi1 Translationally Regulates Mammalian numb Gene Expression by Interacting with Its mRNA
The results suggested that Msi1 could regulate the expression of its target gene at the translational level, and it was found that Notch signaling activity was increased byMsi1 expression in connection with the posttranscriptional down-regulation of them-numb gene.
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
There continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes, so it is important to update guidelines for monitoring autophagic activity in different organisms.
The Wnt/calcium pathway activates NF-AT and promotes ventral cell fate in Xenopus embryos
Data indicate that XNF-AT functions as a downstream target of the Wnt/Ca2+ and Ins(1,4,5)P3- Ca2+ pathways, and has an essential role in mediating ventral signals in the Xenopus embryo through suppression of the canonical Wnt pathway.
‘Ischemic tolerance’ phenomenon found in the brain
Scrambler and yotari disrupt the disabled gene and produce a reeler -like phenotype in mice
The similar phenotypes of reeler, scrambler, yotari and mdab1 null mice indicate that Reelin and mDab1 function as signalling molecules that regulate cell positioning in the developing brain.
Astrocyte Calcium Signaling Transforms Cholinergic Modulation to Cortical Plasticity In Vivo
It is demonstrated that astrocyte Ca2+ signaling provides a critical bridge between cholinergic activation, associated with attention and vigilance states, and somatosensory plasticity in mouse barrel cortex in vivo.
Requirement of the inositol trisphosphate receptor for activation of store-operated Ca2+ channels.
- H. Ma, R. Patterson, D. V. van Rossum, L. Birnbaumer, K. Mikoshiba, D. Gill
- 3 March 2000
It is concluded that insP3Rs mediate both SOC and TRP channel opening and that the InsP3R is essential for maintaining coupling between store emptying and physiological activation of SOCs.