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Epoxide electrophiles as activity-dependent cysteine protease profiling and discovery tools.
A simple and flexible method for functionally identifying cysteine proteases while simultaneously tracking their relative activity levels in crude protein mixtures is created and information obtained from libraries of affinity probes provides a rapid method for obtaining detailed functional information without the need for prior purification/identification of targets.
The characteristics of peptide collision-induced dissociation using a high-performance MALDI-TOF/TOF tandem mass spectrometer.
A new matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) high-resolution tandem mass spectrometer is described for sequencing peptides. This instrument
A heteromeric Texas coral snake toxin targets acid-sensing ion channels to produce pain
A mechanism whereby snake venoms produce pain is revealed, and an unexpected contribution of ASIC1 channels to nociception is highlighted, raising the possibility that ASIC channels function as coincidence detectors for extracellular protons and other, as yet unidentified, endogenous factors.
Proteomic Analysis of Schistosoma mansoni Cercarial Secretions*S
Identifying proteins released by cercariae when they are experimentally induced to exhibit invasive behavior provides important new information for validation of models of skin invasion and immune evasion and aids in rational development of an anti-schistosome vaccine.
O-Linked N-Acetylglucosamine Proteomics of Postsynaptic Density Preparations Using Lectin Weak Affinity Chromatography and Mass Spectrometry*S
Methods for direct enrichment and identification of in vivo O-GlcNAc-modified peptides through lectin weak affinity chromatography (LWAC) and mass spectrometry are reported and suggest specific roles for O- GlcNAC modification in synaptic transmission, establish a basis for site-specific regulatory studies, and provide methods that will facilitate O- GloverNAc proteome analysis across a wide variety of cells and tissues.
An antibiotic factory caught in action
The 2.0-Å structure of the actinorhodin KS-CLF is presented, which shows polyketides being elongated inside an amphipathic tunnel ∼17 Å in length at the heterodimer interface and provides evidence that the first cyclization of the polyketide occurs within the KS- CLF tunnel.
In-depth Analysis of Tandem Mass Spectrometry Data from Disparate Instrument Types*S
New software within Protein Prospector is presented that allows comprehensive analysis of data sets by analyzing the data at increasing levels of depth, and several modifications never previously reported in proteomics data were identified in these standard data sets using this mass modification searching approach.
Arabidopsis PPR40 Connects Abiotic Stress Responses to Mitochondrial Electron Transport1[W][OA]
A mitochondrial pentatricopeptide repeat (PPR) domain protein, PPR40, is described, which provides a signaling link between mitochondrial electron transport and regulation of stress and hormonal responses in Arabidopsis, suggesting a close link between regulation of oxidative respiration and environmental adaptation inArabidopsis.
Comprehensive Analysis of a Multidimensional Liquid Chromatography Mass Spectrometry Dataset Acquired on a Quadrupole Selecting, Quadrupole Collision Cell, Time-of-flight Mass Spectrometer
The results of this comprehensive dataset study demonstrate that QqTOF instruments produce information-rich data of which a high percentage of the data is readily interpretable.
Low-mass ions produced from peptides by high-energy collision-induced dissociation in tandem mass spectrometry
A database consisting of 228 high-energy CID spectra of peptides has been established, and the frequency of occurrence of various ions indicative of specific ammo acid residues has been determined.