Epoxide electrophiles as activity-dependent cysteine protease profiling and discovery tools.
- D. Greenbaum, K. Medzihradszky, A. Burlingame, M. Bogyo
- BiologyChemistry and Biology
- 1 August 2000
A heteromeric Texas coral snake toxin targets acid-sensing ion channels to produce pain
- Christopher J. Bohlen, A. Chesler, D. Julius
- BiologyNature
- 2 November 2011
A mechanism whereby snake venoms produce pain is revealed, and an unexpected contribution of ASIC1 channels to nociception is highlighted, raising the possibility that ASIC channels function as coincidence detectors for extracellular protons and other, as yet unidentified, endogenous factors.
The characteristics of peptide collision-induced dissociation using a high-performance MALDI-TOF/TOF tandem mass spectrometer.
- K. Medzihradszky, J. Campbell, A. Burlingame
- ChemistryAnalytical Chemistry
- 1 February 2000
A new matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) high-resolution tandem mass spectrometer is described for sequencing peptides. This instrument…
Proteomic Analysis of Schistosoma mansoni Cercarial Secretions*S
- G. Knudsen, K. Medzihradszky, K. Lim, E. Hansell, J. McKerrow
- BiologyMolecular & Cellular Proteomics
- 1 December 2005
Identifying proteins released by cercariae when they are experimentally induced to exhibit invasive behavior provides important new information for validation of models of skin invasion and immune evasion and aids in rational development of an anti-schistosome vaccine.
O-Linked N-Acetylglucosamine Proteomics of Postsynaptic Density Preparations Using Lectin Weak Affinity Chromatography and Mass Spectrometry*S
- K. Vosseller, J. Trinidad, A. Burlingame
- Biology, ChemistryMolecular & Cellular Proteomics
- 1 May 2006
Methods for direct enrichment and identification of in vivo O-GlcNAc-modified peptides through lectin weak affinity chromatography (LWAC) and mass spectrometry are reported and suggest specific roles for O- GlcNAC modification in synaptic transmission, establish a basis for site-specific regulatory studies, and provide methods that will facilitate O- GloverNAc proteome analysis across a wide variety of cells and tissues.
In-depth Analysis of Tandem Mass Spectrometry Data from Disparate Instrument Types*S
- R. Chalkley, P. Baker, K. Medzihradszky, A. Lynn, A. Burlingame
- ChemistryMolecular & Cellular Proteomics
- 1 December 2008
New software within Protein Prospector is presented that allows comprehensive analysis of data sets by analyzing the data at increasing levels of depth, and several modifications never previously reported in proteomics data were identified in these standard data sets using this mass modification searching approach.
An antibiotic factory caught in action
- A. Keatinge-Clay, D. Maltby, K. Medzihradszky, C. Khosla, R. Stroud
- ChemistryNature Structural &Molecular Biology
- 1 August 2004
The 2.0-Å structure of the actinorhodin KS-CLF is presented, which shows polyketides being elongated inside an amphipathic tunnel ∼17 Å in length at the heterodimer interface and provides evidence that the first cyclization of the polyketide occurs within the KS- CLF tunnel.
Signaling to Transcription Networks in the Neuronal Retrograde Injury Response
- I. Michaelevski, Y. Segal-Ruder, M. Fainzilber
- BiologyScience Signaling
- 13 July 2010
The data indicate that nerve injury responses are controlled by multiple regulatory components, and suggest that network redundancies provide robustness to the injury response, as well as suggesting the role of transcription factors implicated in the sensory neuron response to axonal injury.
Comprehensive Analysis of a Multidimensional Liquid Chromatography Mass Spectrometry Dataset Acquired on a Quadrupole Selecting, Quadrupole Collision Cell, Time-of-flight Mass Spectrometer
- R. Chalkley, P. Baker, A. Burlingame
- PhysicsMolecular & Cellular Proteomics
- 1 August 2005
The results of this comprehensive dataset study demonstrate that QqTOF instruments produce information-rich data of which a high percentage of the data is readily interpretable.
Medicago truncatula symbiotic peptide NCR247 contributes to bacteroid differentiation through multiple mechanisms
- A. Farkas, G. Maróti, E. Kondorosi
- BiologyProceedings of the National Academy of Sciences
- 25 March 2014
The work reveals a complex interaction network of NCR247, which affects bacterial cell division machinery, translation, and protein folding, and gives an insight how the host can modulate the physiology of the endosymbionts and may serve as a paradigm for other symbiotic systems.
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