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Artificial dirt: microfluidic substrates for nematode neurobiology and behavior.
TLDR
A new class of microfluidic devices for C. elegans neurobiology and behavior are presented: agarose-free, micron-scale chambers and channels that allow the animals to crawl as they would on agarOSE, likely to accelerate studies of the neuronal basis of behavior in this organism.
An Image-Free Opto-Mechanical System for Creating Virtual Environments and Imaging Neuronal Activity in Freely Moving Caenorhabditis elegans
TLDR
A system that tracks neuron-sized fluorescent targets in real time is devised that can be used to create virtual environments by optogenetic activation of sensory neurons, or to image activity in identified neurons at high magnification, and test the long-standing hypothesis that forward and reverse locomotion are generated by distinct neuronal circuits.
Microfluidic Devices for Analysis of Spatial Orientation Behaviors in Semi-Restrained Caenorhabditis elegans
TLDR
The fabrication and use of microfluidic devices for investigating spatial orientation behaviors in nematode worms are described, identifying previously uncharacterized aspects of the behavioral mechanisms underlying chemotaxis, osmotic avoidance, and thermotaxis in this organism.
Circuit and Behavioral Analysis of Klinotaxis in Caenorhabditis elegans
TLDR
This dissertation investigates the neural basis of the sensorimotor transformation underlying a spatial orientation strategy in the nematode Caenorhabditis elegans and finds a minimal neuronal network for klinotaxis to sodium chloride including the ASE, AIY, AIZ, and SMB neuron classes that displays left/right asymmetry across the sensory neuron, interneuron, and motor neuron levels.
Activation of two forms of locomotion by a previously identified trigger interneuron for swimming in the medicinal leech
TLDR
It is shown that stimulation of cell Tr1, a previously identified trigger interneuron for swimming in the medicinal leech, can also elicit the motor program for crawling in isolated nerve cords, and that motor choice is independent of the firing frequency of Tr1 and amount of spiking activity.
A Gene Replacement Humanization Platform for Rapid Functional Testing of Clinical Variants in Epilepsy-associated STXBP1
TLDR
It is demonstrated that automated analysis of a small animal system is an effective, scalable, and fast way to understand functional consequences of variants in STXBP1, one of the most common causes of genetic epilepsies and neurodevelopmental disorders.
Reduced pharyngeal pumping rates observed in tph-1 mutants using microfluidic electropharyngeogram (EPG) recordings
TLDR
Pharyngeal pumping was measured in Caenorhabditis elegans during this elevated feeding phase due to the hypothesis that tph-1 animals would exhibit lower pumping rates than control worms.