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Optimization of Protease Secretion in Bacillus subtilis and Bacillus licheniformis by Screening of Homologous and Heterologous Signal Peptides
ABSTRACT Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture mediumExpand
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The extracellular proteome of Bacillus licheniformis grown in different media and under different nutrient starvation conditions
The now finished genome sequence of Bacillus licheniformis DSM 13 allows the prediction of the genes involved in protein secretion into the extracellular environment as well as the prediction of theExpand
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Directed evolution of a bacterial α‐amylase: Toward enhanced pH‐performance and higher specific activity
α‐Amylases, in particular, microbial α‐amylases, are widely used in industrial processes such as starch liquefaction and pulp processes, and more recently in detergency. Due to the need forExpand
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A proteomic view of cell physiology of Bacillus licheniformis
The still ongoing sequencing of Bacillus licheniformis at the Göttingen Sequencing Laboratory provides the basis for proteome studies of the bacterium. By using two‐dimensional (2‐D) electrophoresisExpand
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An efficient transformation method for Bacillus subtilis DB104
Bacillus subtilis strains are used for extracellular expression of enzymes (i.e., proteases, lipases, and cellulases) which are often engineered by directed evolution for industrial applications. B.Expand
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Surface charge engineering of a Bacillus gibsonii subtilisin protease
In proteins, a posttranslational deamidation process converts asparagine (Asn) and glutamine (Gln) residues into negatively charged aspartic (Asp) and glutamic acid (Glu), respectively. This processExpand
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Stress Responses of the Industrial Workhorse Bacillus licheniformis to Osmotic Challenges
The Gram-positive endospore-forming bacterium Bacillus licheniformis can be found widely in nature and it is exploited in industrial processes for the manufacturing of antibiotics, specialtyExpand
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Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution
Bacillus gibsonii Alkaline Protease (BgAP) is a recently reported subtilisin protease exhibiting activity and stability properties suitable for applications in laundry and dish washing detergents.Expand
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The glucose and nitrogen starvation response of Bacillus licheniformis
The glucose and nitrogen starvation stimulons of Bacillus licheniformis were determined by transcriptome and proteome analyses. Under both starvation conditions, the main response of B. licheniformisExpand
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The phosphate‐starvation response of Bacillus licheniformis
The phosphate‐starvation stimulon of Bacillus licheniformis was analyzed at the transcriptional and translational level. The comparison of the transcriptome and the proteome demonstrated that thisExpand
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