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Purification and characterization of perlucin and perlustrin, two new proteins from the shell of the mollusc Haliotis laevigata.
Two new proteins, named perlucin and perlustrin, with M(r) 17,000 and 13,000, respectively, were isolated from the shell of the mollusc Halotis laevigata (abalone) by ion-exchange chromatography andExpand
Structure, function and tissue forms of the C‐terminal globular domain of collagen XVIII containing the angiogenesis inhibitor endostatin
The C‐terminal domain NC1 of mouse collagen XVIII (38 kDa) and the shorter mouse and human endostatins (22 kDa) were prepared in recombinant form from transfected mammalian cells. The NC1 domainExpand
Recombinant nidogen consists of three globular domains and mediates binding of laminin to collagen type IV.
Recombinant mouse nidogen and two fragments were produced in mammalian cells and purified from culture medium without resorting to denaturing conditions. The truncated products were fragments Nd‐IExpand
The chicken egg white proteome
  • K. Mann
  • Biology, Medicine
  • Proteomics
  • 1 October 2007
Using 1‐D PAGE and LC‐MS/MS and MS3 we identified 78 chicken egg white proteins, 54 of which were identified in egg white for the first time. All proteins were quantitated by calculating theirExpand
Structural and Functional Analysis of the Recombinant G Domain of the Laminin α4 Chain and Its Proteolytic Processing in Tissues*
The C-terminal G domains of laminin α chains have been implicated in various cellular and other interactions. The G domain of the α4 chain was now produced in transfected mammalian cells as twoExpand
The amino-acid sequence of the abalone (Haliotis laevigata) nacre protein perlucin. Detection of a functional C-type lectin domain with galactose/mannose specificity.
Perlucin isolated from abalone nacre consists of 155 amino acids including a glycosylated asparagine. The sequence of the first 130 amino acids shows a high similarity to the C-typeExpand
Proteomic analysis of the acid‐soluble organic matrix of the chicken calcified eggshell layer
The major difference between inorganic minerals and biominerals is the presence of an organic matrix consisting of proteins, glycoproteins, proteoglycans, and polysaccharides, which is synthesized byExpand
Mip protein of Legionella pneumophila exhibits peptidyl‐prolyl‐cis/trans isomerase (PPIase) activity
Legionella pneumophila is an intracellular parasite which is able to survive and multiply in human monocytes and alveolar macrophages. The Mip (macro‐phage infectivity potentiator) protein has beenExpand
Perlustrin, a Haliotis laevigata (abalone) nacre protein, is homologous to the insulin-like growth factor binding protein N-terminal module of vertebrates.
The 84-amino-acid-long sequence of perlustrin showed homology of the abalone nacre protein to the N-terminal domain of mammalian insulin-like growth factor binding proteins (IGFBPs). Despite theExpand
The eggshell: structure, composition and mineralization.
The calcareous egg is produced by all birds and most reptiles. Current understanding of eggshell formation and mineralization is mainly based on intensive studies of one species - the domesticatedExpand