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Complete Reversal of Coenzyme Specificity of Xylitol Dehydrogenase and Increase of Thermostability by the Introduction of Structural Zinc*
The introduction of three cysteine residues in wild-type XDH gave an additional zinc-binding site and improved the thermostability and further increased the catalytic activity with NADP+. Expand
Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein-engineered NADH-preferring xylose reductase from Pichia stipitis.
Measurement of intracellular coenzyme concentrations suggested that maintenance of the of NADPH/NadP(+) and NADH/NAD(+) ratios is important for efficient ethanol fermentation from xylose by recombinant S. cerevisiae. Expand
A novel method for synthesis of silica nanoparticles.
The results obtained in the present study are in agreement with the results observed for the electronic absorption behavior of silica particles, which was measured by UV-vis spectrophotometry. Expand
Cloning, Expression, and Characterization of Bacterial l-Arabinose 1-Dehydrogenase Involved in an Alternative Pathway of l-Arabinose Metabolism*
Results indicated that the enzyme is involved in the metabolism of l-arabinose but not d-galactose, the first identification of a gene involved in an alternative pathway of l -arabinOSE metabolism in bacterium. Expand
Eukaryotic and Bacterial Gene Clusters Related to an Alternative Pathway of Nonphosphorylated L-Rhamnose Metabolism*
Substrate specificity and kinetic analysis revealed that nonphosphorylated intermediates related to l-rhamnose are significant active substrates for the purified LRA1-4 proteins. Expand
Homologous Recombination but Not Nucleotide Excision Repair Plays a Pivotal Role in Tolerance of DNA-Protein Cross-links in Mammalian Cells*
It is shown that the upper size limit of CLPs amenable to mammalian NER is relatively small so that NER cannot participate in the repair of chromosomal DPCs in mammalian cells, and the versatile and conserved role of HR in tolerance of D PCs among species is demonstrated. Expand
Identification and Characterization of l-Arabonate Dehydratase, l-2-Keto-3-deoxyarabonate Dehydratase, and l-Arabinolactonase Involved in an Alternative Pathway of l-Arabinose Metabolism
Genetic characterization of the alternative l-arabinose pathway suggested a significant evolutional relationship with the known sugar metabolic pathways, including the Entner-Doudoroff (ED) pathway and the several modified versions. Expand
Synthesis and characterization of a practically better DEPMPO-type spin trap, 5-(2,2-dimethyl-1,3-propoxy cyclophosphoryl)-5-methyl-1-pyrroline N-oxide (CYPMPO)
Although overall spin-trapping capabilities of CYPMPO are similar to DEPMPO, its high melting point, low hygroscopic property, and the long shelf-life would be highly advantageous for the practical use. Expand
Nucleotide excision repair and homologous recombination systems commit differentially to the repair of DNA-protein crosslinks.
The roles of nucleotide excision repair (NER) and homologous recombination (HR) in the repair of DPCs both in vitro and in vivo using a bacterial system are analyzed to highlight how NER and HR are coordinated when cells need to deal with unusually bulky DNA lesions such as D PCs. Expand
Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein engineered NADP+-dependent xylitol dehydrogenase.
Effects of reversal coenzyme specificity toward NADP+ and thermostabilization of xylitol dehydrogenase (XDH) from Pichia stipitis on fermentation of xylose to ethanol were estimated using aExpand